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AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189923

Purpose

AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

nSaCas9 KKH, TadA ABE8e V106W

Promoter

EFS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

Plasmid

#201916

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-Cas9 v2
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Expression

Mammalian

Promoter

CAG and Human U6

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CBh; U6

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_NSlmb-vhhGFP4

Plasmid

#35579

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Mammalian

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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p11-LacY-wtx1

Plasmid

#69056

Purpose

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

Depositor

Huimin Zhao

Article

Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

Inserts

ccdB (ccdB )
lacY (lacY )

Tags

A177C

Promoter

pBAD and pLac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

Plasmid

#216156

Purpose

Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with C-terminal FLAG tag

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNF02-mLemon

Plasmid

#219399

Purpose

Constitutive expression of mLemon in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLemon

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mycBioID

Plasmid

#35700

Purpose

To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA

Tags

Myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

cmv

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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pRN112

Plasmid

#84464

Purpose

pJB38-NWMN29-30+ SarA_P1-mAmetrine-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mAmetrine from pRN12

Use

Synthetic Biology

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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