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ECFP-ER-0

Plasmid

#55342

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

ER (CALR Human)

Tags

ECFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
GB4542

Plasmid

#215261

Purpose

Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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GB4401

Plasmid

#215252

Purpose

Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-Nb80

Plasmid

#205519

Purpose

Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-Nb80

Tags

Nuclear localization signal (NLS) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDN80

Plasmid

#61349

Purpose

LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Type

Empty backbone

Tags

AsLOV2 wt and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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Twitch-1 pcDNA3

Plasmid

#49812

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-1

Tags

ECFP and cpCit174

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
B2094_CRY2olig-miRFP

Plasmid

#215114

Purpose

Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CRY2olig-miRFP (CRY2 Mustard Weed)

Tags

miRFP

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDBTrp-LexABD-CRY2FL

Plasmid

#78210

Purpose

Use with Addgene 40615 for blue light transcription control in yeast

Depositor

Chandra Tucker

Article

Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

Insert

LexABD-CRY2 (CRY2 Mustard Weed)

Tags

lexA binding domain

Expression

Yeast

Mutation

contains endogenous CRY2 NLS

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCHC005 (∆RE)

Plasmid

#226199

Purpose

Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for type I restriction-modification system restriction subunit H16_A0006

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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dmBACCS2-IRES-dOrai

Plasmid

#67627

Purpose

Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2-IRES-dOrai

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-JEDI-1P-WPRE

Plasmid

#202615

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b/bPanK

Plasmid

#73619

Purpose

Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

Depositor

Suzanne Jackowski, T. J. Kappock

Article

Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

Insert

pantothenate kinase (coaA Escherichia coli)

Tags

His6

Expression

Bacterial

Mutation

relative to MG1655 sequence, changes glutamate-5 …

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCHC039 (∆phcA)

Plasmid

#226207

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR223-BMRF1(EA-D)

Plasmid

#195807

Purpose

Gateway Entry Clone of Epstein-Barr virus BMRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BMRF1 (BMRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pOG-Cre

Plasmid

#233265

Purpose

Plasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

Cre recombinase

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pTU398

Plasmid

#178980

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

gRAMP

Tags

Strep-Tag II SUMO

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP11H

Plasmid

#188063

Purpose

PROSS-designed versatile peroxidase 11H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

38 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR223-BZLF2

Plasmid

#195784

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF2 (BZLF2 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-EBNALP

Plasmid

#195817

Purpose

Gateway Entry Clone of Epstein-Barr virus EBNALP

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

EBNALP (EBNA-LP Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Cx32-7

Plasmid

#55287

Purpose

Localization: Gap Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Cx32 (GJB1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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