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Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR1a

Plasmid

#17926

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)

Tags

eGFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1:ChrH-ChrI

Plasmid

#225083

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SynB

Plasmid

#174861

Purpose

Expresses mouse syncytin B in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin B (Synb Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pN006

Plasmid

#188470

Purpose

Lentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

AsCas12a

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SynA

Plasmid

#174860

Purpose

Expresses mouse syncytin A in mammalian cells

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

syncytin A (Syna Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin TS

Plasmid

#68127

Purpose

nesprin tension sensor

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

mouse nesprin mini 2G (Syne2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_GCAMP7s

Plasmid

#182940

Purpose

alpha7 nACHR expression in mammalian cell lines with calcium reporter

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHS2002

Plasmid

#239832

Purpose

Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB omegaRNA scaffold

Expression

Mammalian

Mutation

Replacement of A12-A57 with GAAA in omegaRNA scaf…

Promoter

human U6

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHS_Nova

Plasmid

#239831

Purpose

NovaIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1 and swap o…

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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LacZ in pLX304

Plasmid

#42560

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

LacZ

Use

Lentiviral

Tags

2xV5

Promoter

CMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cre)

Plasmid

#174862

Purpose

Expresses mouse Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by MmPeg10 UTRs

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMIIS793

Plasmid

#238206

Purpose

Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

TwinStrepII-bdSUMO

Expression

Bacterial

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE NFKB-TA-LUC-UBC-dTomato-W

Plasmid

#49335

Purpose

pHAGE NFKB-TA-LUC-UBC-dTomato-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

NFkB consensus binding sequence
TA
Luciferase
UBC
dTomato

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHS1742

Plasmid

#239838

Purpose

OrufIscB-KRK mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-KRK

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1 + E137K/E4…

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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HA-hULK1

Plasmid

#31963

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

Plasmid

#229227

Purpose

Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

Depositor

Willow Coyote-Maestas

Article

Howard et al Cell. 2025 Feb 6;188(3):671-687.e20. doi

Type

Empty backbone

Tags

mNeonGreen11

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Article

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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