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pnEA-NpM-DmThor_50-83_Z

Plasmid

#148179

Purpose

Bacterial Expression of DmThor_50-83

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor_50-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_63-83-GB1_Z

Plasmid

#148181

Purpose

Bacterial Expression of DmThor_63-83

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor_63-83 (Thor Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-del28-43-V5His6_Z

Plasmid

#148157

Purpose

Insect Expression of Dm4E-T_1-511-del28-43

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-del28-43 (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-FWKtoAAA-V5His6_Z

Plasmid

#148158

Purpose

Insect Expression of Dm4E-T_1-511-FWKtoAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-FWKtoAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-L36A L69A-V5His6_Z

Plasmid

#148159

Purpose

Insect Expression of Dm4E-T_1-511-L36AL69A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-L36AL69A (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-Y10AL15A-V5His6_Z

Plasmid

#148161

Purpose

Insect Expression of Dm4E-T_1-511-Y10AL15A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-Y10AL15A (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4E-T_1-511-YLLLtoAAAA-V5His6_Z

Plasmid

#148162

Purpose

Insect Expression of Dm4E-T_1-511-YLLLtoAAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T_1-511-YLLLtoAAAA (4E-T Fly)

Expression

Insect

Mutation

two non silent mutations L127S, annotated as EST,…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417-CG32016-E_T

Plasmid

#147583

Purpose

Insect Expression of DmCup_1-417-CG32016-E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCup_1-417-CG32016-E (cup Fly)

Expression

Insect

Mutation

5 silent and three no silent mutations H117R, N14…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-DmCup_417-1133_T

Plasmid

#147589

Purpose

Insect Expression of DmeIF4Ga_578-650-DmCup_417-1133

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4Ga_578-650-DmCup_417-1133 (eIF4G1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I

Plasmid

#146577

Purpose

Insect Expression of DmeIF4Ga

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4Ga (eIF4G1 Fly)

Expression

Insect

Mutation

one non silent mutation K340R compared to the seq…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4G_578-634-GB1_AG

Plasmid

#148744

Purpose

Bacterial Expression of DmeIF4G_578-634

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_578-634 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTV-Flnc-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174881

Purpose

Targeting vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Flnc

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Usp5-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174885

Purpose

Targeting vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Usp5

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Wnk1-5prime-1000-24MS96T-3F_NeoR

Plasmid

#174886

Purpose

Targeting vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

STREAMING-tag with homology arms for Wnk1

Use

Mouse Targeting

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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TERTp124_Gluc_CCR5

Plasmid

#192271

Purpose

Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-124 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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TERTp146_Gluc_CCR5

Plasmid

#192272

Purpose

Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-146 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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TOE1

Plasmid

#156091

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TOE1 (TOE1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RBM41

Plasmid

#155902

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM41 (RBM41 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRB116.2

Plasmid

#181946

Purpose

aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

TorR-mNG; mCherry; tetR (AX04_RS23485, torR Synthetic, torR - E. coli)

Tags

mNeonGreen

Expression

Bacterial

Promoter

TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRB120.2

Plasmid

#181947

Purpose

aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNG-TorR; mCherry; tetR (torR, AX04_RS23485 Synthetic, torR - E. coli)

Tags

mNeonGreen

Expression

Bacterial

Promoter

mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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