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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEE515

Plasmid

#149288

Purpose

T-DNA encoding TRV2 complete genome with MCS

Depositor

Daniel Voytas

Publication

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Type

Empty backbone

Expression

Plant

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT3-EF1A-mTert

Plasmid

#162555

Purpose

Overexpression of Tert

Depositor

Amaia Lujambio

Publication

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of Tert (Tert Mouse)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163029

Purpose

FLiCRE TF for neuronal expression

Depositor

Alice Ting

Publication

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Publication

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pNCST-AausFP1

Plasmid

#129499

Purpose

Expresses AausFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Publication

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSLIK CA Rac1

Plasmid

#84605

Purpose

Lentiviral expression of doxycycline-inducible constitutively active Rac1 and co-expression of Venus

Depositor

Sanjay Kumar

Publication

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pOPINE-3C-eGFP

Plasmid

#41125

Depositor

Ray Owens

Insert

GFP

Expression

Bacterial, Insect, and Mamm…

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcdna3 flag - ULK2 wt

Plasmid

#27637

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK2 (Ulk2 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M removed from ULK2.

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCDNA3-myc3-FBW5

Plasmid

#19905

Depositor

Yue Xiong

Publication

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMyC-kan

Plasmid

#84692

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Publication

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-AURKA

Plasmid

#23532

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AURKA (AURKA Human)

Use

Gateway Cloning

Mutation

I57V

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pTKW106alp7A

Plasmid

#69360

Purpose

Stabilized lacZ expression vector containing alp7A and hok/sok

Depositor

Jeff Hasty

Publication

Danino et al Sci Transl Med. 2015 May 27;7(289):289ra

Inserts

lacZ
alp7A
hok
placIq-lacI

Expression

Bacterial

Promoter

pTac and placIq

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFasBac+GFP-CAMSAP1

Plasmid

#59036

Purpose

for baculovirus expression of CAMSAP1 in Sf9 cells

Depositor

Ron Vale

Publication

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 (CAMSAP1 Human)

Tags

His and eGFP

Expression

Insect

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMZ379

Plasmid

#74215

Purpose

Combined sgRNA/Cas9 vector with NatMX marker

Depositor

Mikel Zaratiegui

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

adh1 and rrk1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAGM8031

Plasmid

#48037

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-Prd

Plasmid

#18038

Depositor

Scot Wolfe

Publication

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

Paired (prd Fly)

Expression

Bacterial

Mutation

Paired is simply used as an insert between Kpn1 a…

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_MDM2_WT

Plasmid

#82897

Purpose

Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MDM2 (MDM2 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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