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MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Plasmid

#135454

Purpose

Bacterial expression of TEVΔ protease

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation improves stability. Truncated form…

Promoter

tac promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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tUI

Plasmid

#122661

Purpose

Express tUI protein in E. coli

Depositor

Robert Cohen

Publication

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tUI (UBQLN1, USP5 Human)

Tags

His-tag

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5 Flag-RagC(S75N)

Plasmid

#99724

Purpose

Mammalian expression of Flag-RagC(S75N)

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

Flag

Mutation

S75N

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Lysosomes-20

Plasmid

#58022

Purpose

Localization: Lysosome Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PLlacO-1-mfLon

Plasmid

#45152

Depositor

Michel Maharbiz

Publication

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lon

Use

Synthetic Biology

Tags

ssRA degradation (LVA)

Expression

Bacterial

Promoter

PLlacO-1

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pMT643

Plasmid

#8610

Depositor

Robert Hartley

Publication

Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

Insert

barstar A

Expression

Bacterial

Mutation

Two cysteines of barstar changed to alanine, C40A…

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pQCXIZ GFP PODXL A WT

Plasmid

#202413

Purpose

Expression of GFP-tagged PODXL (isoform A) WT

Depositor

David Bryant

Publication

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

PODXL (PODXL Human)

Use

Lentiviral

Tags

GFP

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKate2-B_UTR

Plasmid

#68480

Purpose

Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKate2-B_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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FLM-PC1

Plasmid

#41567

Depositor

Thomas Weimbs

Publication

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PKD1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

sequence from 4078 - 4303 of PKD1

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223-CSNK1G3

Plasmid

#23400

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK1G3 (CSNK1G3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3C3

Plasmid

#23457

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3C3 (PIK3C3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PI4K2B

Plasmid

#23507

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4K2B (PI4K2B Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Publication

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Publication

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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LifeAct-mhYFP

Plasmid

#186524

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to actin

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Publication

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Expression

Yeast

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE Hygro ST6GalNaC5

Plasmid

#26981

Depositor

Joan Massague

Publication

Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

Insert

ST6GalNaC5 (ST6GALNAC5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-TAOK3

Plasmid

#23861

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TAOK3 (TAOK3 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MAST2

Plasmid

#23456

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAST2 (MAST2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.iGluSnFR3.v857.SGZ

Plasmid

#178334

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Publication

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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