We narrowed to 4,495 results for: Ado

1. EF1a_KLF17_P2A_Hygro_Barcode

   Plasmid

   #120503

   Purpose

   Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF17 (KLF17 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a_SOX2_P2A_Hygro_Barcode

   Plasmid

   #120480

   Purpose

   Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-nEF-dCas9

   Plasmid

   #106430

   Purpose

   Expresses SpdCas9 from the nEF promoter in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   nEF-dCas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSynapsin1.OxLight-ctr

   Plasmid

   #169793

   Purpose

   Control non-binding fluorescent reporter for orexin neuropeptides

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

   Insert

   OxLight-ctr
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E54K; T111A

   Promoter

   human Synapsin-1

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK092

   Plasmid

   #183146

   Purpose

   pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CAG-ChR2-YFP

   Plasmid

   #204345

   Purpose

   PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   ChR2-YFP
   

   Use

   Tags

   Yellow fluorescent protein

   Expression

   Mammalian

   Mutation

   C-terminus (aa315-737) is deleted - PMID: 1461559…

   Promoter

   CAG

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEZYeGFP-SARS-CoV-2E

   Plasmid

   #224580

   Purpose

   To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

   Depositor

   Isabel Mérida

   Article

   Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

   Insert

   SARS-CoV-2E (E SARS-CoV-2)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_ETV2_P2A_Hygro_Barcode

   Plasmid

   #120436

   Purpose

   Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   ETV2 (ETV2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a_TFAP2C_P2A_Hygro_Barcode

    Plasmid

    #120487

    Purpose

    Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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