We narrowed to 6,927 results for: 11

1. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

   Plasmid

   #107942

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
   

   Use

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R, and N282A

   Promoter

   CAG

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-CMV-Puro-PNPT1

   Plasmid

   #223315

   Purpose

   Lentiviral vector expressing human PNPT1

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV uN2CpolyGly op GFP (NIID)

   Plasmid

   #224356

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C with a GGC repeat expansion (100x)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intron (CAG)

   Available since

   Feb. 5, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. lentiCRISPR v2-sgPnpt1-2#

   Plasmid

   #234770

   Purpose

   knockout Pnpt1

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Pnpt1 (Pnpt1 )
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-MH6-uvsY

   Plasmid

   #163914

   Purpose

   Expresses uvsY for bacterial expression and affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   uvsY (uvsY Enterobacteria phage T4)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

   Plasmid

   #62723

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1 and AAV5

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

   Plasmid

   #114073

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   E174R, S542R, K548R and N282A

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR 223 VPS4A

   Plasmid

   #169319

   Purpose

   Gateway entry vector for human VPS4A ORF

   Depositor

   Francisca Vazquez

   Article

   Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   NA

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS SARS-CoV-2 Delta Spike

    Plasmid

    #185593

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 Delta Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 Delta Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19R, Δ156-157,…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid

    #114084

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.4

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

    Plasmid

    #84483

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    human synapsin promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid

    #114082

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    

    Use

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR 223 VPS4B

    Plasmid

    #169320

    Purpose

    Gateway entry vector for human VPS4B ORF

    Depositor

    Francisca Vazquez

    Article

    Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

    Insert

    VPS4B (VPS4B Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSPL3-hRPH3A_c.1853Gmut

    Plasmid

    #190479

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.1853A>G

    Promoter

    SV40

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS SARS-CoV-2 Alpha Spike

    Plasmid

    #185691

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 Alpha Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: Δ69-70, Δ145, N…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #128587

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

    Plasmid

    #114081

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

    Plasmid

    #159630

    Purpose

    AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

    Depositor

    Andrew Allen, Ross Bathgate

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    Ast (AstA Fly)
    

    Use

    AAV

    Tags

    HA, IRES, mCherry, and neurophysin-1

    Expression

    Mammalian

    Mutation

    Promoter

    DCA (same as CAG promoter)

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. anti-Jagged_D5-pBIOCAM5

    Plasmid

    #39345

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Insert

    scFv specific for Jagged (clone D5) (JAG1 Human)
    

    Use

    Tags

    3xFLAG and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. anti-Desmin_D7-pBIOCAM5

    Plasmid

    #39346

    Depositor

    John McCafferty

    Article

    Schofield et al Genome Biol. 2007;8(11):R254.

    Insert

    scFv-Fc-fusion specific for Desmin (clone D7) (DES Human)
    

    Use

    Tags

    3xFLAG, 6xHis, and human Fc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid

    #114075

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R, S542R, K548V and N552R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEAHISPApqsE

    Plasmid

    #97019

    Purpose

    Expressing Pseudomonas aeruginosa pqsE protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Quinolone signal response protein
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEAHISMRSA2676

    Plasmid

    #96970

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 2676

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Sar2676
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEAHISMRSAMVAK

    Plasmid

    #97000

    Purpose

    expression of methicillin-resistant Staphylococcus aureus MVAK protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Mevalonate kinase
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEAHISPAFabH

    Plasmid

    #97018

    Purpose

    Expressing Pseudomonas aeruginosa FabH protein

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    3-Oxoacyl-[acyl-carrier-protein] synthase III
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. lentiCRISPR v2-sgPnpt1-1#

    Plasmid

    #234769

    Purpose

    knockout Pnpt1

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Pnpt1 (Pnpt1 )
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiCRISPR v2-sgPlscr3-2#

    Plasmid

    #234772

    Purpose

    knockout Plscr3

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Plscr3 (Plscr3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lentiCRISPR v2-sgPlscr3-1#

    Plasmid

    #234767

    Purpose

    knockout Plscr3

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Plscr3 (Plscr3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRSF_his6_sumo_ZIKV_NS5_MTase

    Plasmid

    #213529

    Purpose

    Expresses the MTase domain of NS5 of ZIKV

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    ZIKA virus NS5 methyltransferase domain
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    MTase domain aa 1-265

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge