We narrowed to 12,321 results for: BASE

1. pST5832

   Plasmid

   #36256

   Depositor

   Carolyn Bertozzi, Jessica Seeliger

   Article

   Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

   Insert

   lacZ
   

   Use

   Synthetic Biology; Mycobacteria replicating vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Modified Hsp60

   Available since

   Nov. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

   Plasmid

   #134863

   Purpose

   Expresses EGFP-tagged endoplasmic reticulum marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   RAMP4 (SERP1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Zeo-ultraID

   Plasmid

   #207704

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. p663-UBC-miniTurbo-V5-CDK13_IDG-K

   Plasmid

   #135266

   Purpose

   Gateway destination clone of CDK13 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK13 (CDK13 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3A9cbetaTCR

   Plasmid

   #69600

   Purpose

   TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

   Depositor

   Francis R. Carbone

   Article

   Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)

   Plasmid

   #70198

   Purpose

   Promoter panel

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJO629

   Plasmid

   #104342

   Purpose

   Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

   Insert

   pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. tCRMod-GGSGGS9

   Plasmid

   #111762

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS9-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXylA-agrCA-I

   Plasmid

   #53437

   Purpose

   xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   agrCA locus
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   "MVQTS" sequence added to the N-terminu…

   Promoter

   PxylA

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. p667-UBC-AKT1-V5-miniTurbo_IDG-K

    Plasmid

    #189889

    Purpose

    Gateway destination clone of AKT1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p663-UBC-miniTurbo-V5-AKT1_IDG-K

    Plasmid

    #189890

    Purpose

    Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. nCas9-NG_P2A-mCherry

    Plasmid

    #219934

    Purpose

    Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    nCas9(D10A)

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNR291

    Plasmid

    #79555

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Inserts

    GFPmut3b
    mTagBFP
    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH-roGFP2-GPXC71S

    Plasmid

    #191698

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    redox(ro)GFP2-GPXC71S
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Peroxidatic Cysteine 71 replaced with Serine

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Synapsin-miRFP670-3XNLS-WPRE

    Plasmid

    #159952

    Purpose

    nucleus marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    miRFP670-3XNLS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC-DEST-hSOX2-EGFP-PuroTK

    Plasmid

    #141026

    Purpose

    Reporter integration

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LT0

    Plasmid

    #210551

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT0::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LT1

    Plasmid

    #210552

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT1::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LT2

    Plasmid

    #210553

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT2::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge