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pCS-lpp0.2-K64A

Plasmid

#201789

Purpose

mutant of PadR; transcriptional regulator can respond to p-coumaric acid

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PadR (padR Bacillus subtilis)

Expression

Bacterial

Mutation

K64A

Available Since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-LumiScarlet

Plasmid

#126623

Purpose

A BRET-based mScarlet-I-LumiLuc fusion for red-shifted bioluminescence emission

Depositor

Huiwang Ai

Article

Yeh et al ACS Chem Biol. 2019 Apr 17. doi: 10.1021

Insert

LumiScarlet

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2D3

Plasmid

#15784

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)

Tags

His

Expression

Bacterial

Mutation

see Depositor Comments below

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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GFP-RPA40

Plasmid

#17658

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA40 (POLR1C Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PsChR2_EYFP

Plasmid

#69057

Purpose

High-efficiency blue-shifted cation channelrhodopsin

Depositor

John Spudich

Article

Govorunova et al J Biol Chem. 2013 Oct 11;288(41):29911-2

Insert

PsChR2

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSYC-187

Plasmid

#74794

Purpose

pCS4+-P2A-mCherry-CAAX

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

P2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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piggyBac-ABE8.20-NG

Plasmid

#226866

Purpose

Expresses dox-inducible ABE8.20-NG for mammalian cells. piggyBac vector

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ABE8.20-NG

Use

Lentiviral

Promoter

Tet-On-TRE3G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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AiRTi

Plasmid

#214068

Purpose

Fluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AiRTX_ctrl

Plasmid

#214069

Purpose

Fluorescent reporter system, NMD negative control. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Remnant of linker sequence present at the end of …

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AiRT

Plasmid

#214067

Purpose

Fluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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RV-L3-HyTK-2L

Plasmid

#11684

Depositor

Geoff Wahl

Article

Wong et al Nucleic Acids Res. 2005 . 33(17):e147.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Expression

Mammalian

Available Since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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pCDF_6xHIS_Csx29

Plasmid

#197613

Purpose

For protein purification of Csx29

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

6-HIS tagged Csx29

Expression

Bacterial

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV.IRES.hCD4

Plasmid

#35712

Depositor

Patricia Ernst

Article

Artinger et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Inserts

IRES
hCD4

Use

Retroviral

Expression

Mammalian

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pExp_CAG:EBFP2

Plasmid

#51790

Purpose

Constitutively expresses blue fluorescent protein, EBFP2

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

EBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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mEosEM-E_N1

Plasmid

#226337

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; empty backbone containing mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB33

Plasmid

#158151

Purpose

Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Cas7-11_crRNA

Plasmid

#197612

Purpose

For protein purification of Cas7-11

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Cas7-11 with crRNA

Expression

Bacterial

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BRIC

Plasmid

#172341

Purpose

AAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-APLO

Plasmid

#112805

Purpose

gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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