We narrowed to 18,427 results for: Met

1. mCardinal-pBAD

   Plasmid

   #54800

   Purpose

   Localization: Protein Expression Vector , Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   6xHis and mCardinal

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3-TDsmURFP-IRES-HO-1

   Plasmid

   #80346

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Expression

   Mammalian

   Promoter

   CMV and IRES

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. p4B5-EGFP

   Plasmid

   #140324

   Purpose

   Detection of Dengue virus infection in live cells

   Depositor

   Alan Rothman

   Article

   Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

   Insert

   DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   2 conservative n.t. differences near 5' end …

   Promoter

   CMV

   Available Since

   May 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Str-KDEL_ManII-SBP-mCherry

   Plasmid

   #65253

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-10xSunTag(SRv1.1-2P)

   Plasmid

   #185801

   Purpose

   one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.1

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-sfGFP-GB1-scAB--ubc-nls-pcp-10xSunTag
   

   Expression

   Mammalian

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSupAR-MbPylRS(DiZPK)

   Plasmid

   #91705

   Purpose

   express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

   Depositor

   P. Chen

   Article

   Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

   Inserts

   pylS
   pylT
   

   Expression

   Bacterial

   Mutation

   T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

   Available Since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. modRNAc0-Cas9-2A-GFP

   Plasmid

   #170181

   Purpose

   IVT template plasmid for making modified Cas9GFP RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9-2A-GFP
   

   Use

   Ivt template plasmid

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_MMP-9-HA

   Plasmid

   #121172

   Purpose

   Expression of mouse MMP9

   Depositor

   Shinwu Jeong, M. Elizabeth Fini

   Article

   Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

   Insert

   MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV immediate/early

   Available Since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRuby2-ZO1-C-14

   Plasmid

   #55916

   Purpose

   Localization: Tight Junctions, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   ZO1 (TJP1 Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

    Plasmid

    #98927

    Purpose

    Cre dependent AAV expression of GFP spaghetti monster myc construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    GFP-smFP-myc
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid

    #84245

    Purpose

    Expresses direct fusion KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    KRAB-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, KRAB, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-pelB-LaM-6

    Plasmid

    #172771

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-6
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1_VNp-mNeongreen

    Plasmid

    #182387

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-His
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB1-moxCerulean3

    Plasmid

    #103975

    Purpose

    Plasmid encoding moxCerulean3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxCerulean3
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

    Plasmid

    #98925

    Purpose

    AAV expression of Ruby2 spaghetti monster FLAG construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    Ruby2-smFP-Flag
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CBA

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1-Nst-CAG-DEST

    Plasmid

    #80489

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

    Plasmid

    #31481

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Traffic Light Reporter 1.1 (Sce target) EF BFP
    

    Use

    Lentiviral

    Mutation

    mCherry contains M9S and M16L to reduce backgroun…

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBluescriptII[attB/Ins1]

    Plasmid

    #74100

    Purpose

    "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Phi C31 attb element
    Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
    

    Expression

    Bacterial

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-10xSunTag(22aa)

    Plasmid

    #215745

    Purpose

    Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    dCas9-10xSunTag
    

    Expression

    Mammalian

    Promoter

    chicken beta-actin promoter

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG_mRuby2_smFP OLLAS

    Plasmid

    #59761

    Purpose

    encodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    mRuby2_smFP_OLLAS
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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