We narrowed to 15,769 results for: LEA

1. pEPOR0SP0013

   Plasmid

   #117521

   Purpose

   Level0 Golden Gate part: CDS, SpCas9-p

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   SpCas9-p (with stop codon)
   

   Use

   CRISPR

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-T5-chi28TAG

   Plasmid

   #188984

   Purpose

   Express chimadanin with TAG at 28 position

   Depositor

   Han Xiao

   Article

   Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

   Insert

   Chimadanin with TAG at 28 position
   

   Expression

   Bacterial

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rag2 T490A

   Plasmid

   #115650

   Purpose

   expression of full length Rag2 with T490A mutation

   Depositor

   Martin Gellert

   Article

   Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

   Insert

   Rag2 (Rag2 Mouse)
   

   Tags

   8XHis-MBP-PreScission

   Expression

   Mammalian

   Mutation

   T490A

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL0M-S-mClover3-EC18157

   Plasmid

   #137076

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mClover3 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-Pol-II 15

   Plasmid

   #35177

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains the C-terminal repeats 16-52

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pExodus

   Plasmid

   #39991

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   CMV, T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. p32m3C Trx-CC

   Plasmid

   #196475

   Purpose

   Protein expression of Thiolredoxin-3C cleavage site-CarHCC

   Depositor

   Fei Sun

   Article

   Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

   Insert

   Trx-CC
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30a-YedK

   Plasmid

   #86884

   Purpose

   Bacterial expression of E. coli SRAP ortholog YedK

   Depositor

   Douglas Feldman

   Article

   Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

   Insert

   YedK (yedK Eschericia coli)
   

   Tags

   6xHis, HA tag, and S-Tag

   Expression

   Bacterial

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-delta8.2R

   Plasmid

   #89706

   Purpose

   Donor plasmid for HIV-1 env- integration into human AAVS1 locus

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

   Insert

   AAVS1-delta8.2R
   

   Use

   Unspecified

   Promoter

   CMV

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-perox-mCer

    Plasmid

    #185603

    Purpose

    Lentiviral construct for expression of mCerulean targeted to peroxisomes

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    perox-mCer
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

    Plasmid

    #52478

    Purpose

    Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    EGFP
    nuclear mTagBFP
    Synaptophysin-TagRFP-T
    

    Use

    Rabies virus

    Tags

    TagRFP-T

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-mCherry-FKBP-TEVp

    Plasmid

    #178867

    Purpose

    Mammalian expression of mCherry-FKBP-TEVprotease(Δ220-242)

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    mCherry-FKBP-TEVprotease(Δ220-242)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT316_pattB-pCA-GFP-pA

    Plasmid

    #52548

    Purpose

    for Phic31 integrase-mediated transgene integration

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Expression

    Mammalian

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFREE2-xCas9-sgRNA

    Plasmid

    #179579

    Purpose

    Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    xCas9
    

    Expression

    Bacterial

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSHS293

    Plasmid

    #87348

    Purpose

    D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/D435C/C574S/E945C

    Promoter

    T7

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-LacI-polylinker

    Plasmid

    #179271

    Purpose

    Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

    Depositor

    Lienhard Schmitz

    Article

    Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

    Type

    Empty backbone

    Tags

    GFP and LacI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLX-UBI-Tred

    Plasmid

    #27246

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    TurboRFP
    

    Use

    Lentiviral

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGM-ENO2-mCherry-YFP

    Plasmid

    #180570

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PB1-AG-FRB

    Plasmid

    #178862

    Purpose

    Mammalian expression of PB1-AzamiGreen-FRB

    Depositor

    Shinya Tsukiji

    Article

    Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

    Insert

    PB1-AzamiGreen-FRB
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGM-ENO2-YFP-mCherry

    Plasmid

    #180569

    Purpose

    Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

    Depositor

    Joel McManus

    Article

    May et al Elife. 2023 May 25;12:e69611. doi: 10.75

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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