We narrowed to 12,112 results for: LEA
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Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSup_35Senh(fwd)_35Spr+synJ
Plasmid#149422PurposePlasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.DepositorInsert35S core enhancer
ExpressionPlantAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mTurquoise2-EC18152
Plasmid#137074PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC65
Plasmid#118865Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mGFPExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY008 (pcDNA3.1-hPbCpf1)
Plasmid#69980PurposeExpresses humanized LPbCpf1DepositorInserthPbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
POPDC2 pEGFP-N2 (1087)
Plasmid#62030Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1600
Plasmid#29274PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNDepositorInsertPle129 (MKI67 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable sinceNov. 4, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-NurRE3
Plasmid#208253PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X Nur response element LUC
TagsNoExpressionMammalianPromoterSV40 early promoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b_Q69L
Plasmid#186272PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
TagsHis-tagged at N terminalExpressionBacterialMutationChanged Q69 into LPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB N-term-mScarlet
Plasmid#169218PurposeTargeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locusDepositorInsertDouble SapI flanked mScarlet
UseTargeting vector backboneAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TVBB N-term-mNeongreen
Plasmid#169226PurposeTargeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locusDepositorInsertDouble SapI flanked mNeongreen
UseTargeting vector backboneAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHFR-mNeon-GluA1
Plasmid#173009PurposeFor light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.DepositorInsertDHFR-mNeon-GluA1
TagsDHFR and mNeonExpressionMammalianPromoterChicken beta actin (CAG)Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF Doc2beta WT GFP
Plasmid#128820PurposeTo visualize Doc2beta WT in PC12 cellsDepositorAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-NONO 4
Plasmid#127652PurposeKnock-out of human NONODepositorInsertNONO sgRNA (NONO Human)
UseLentiviralAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluSnFR
Plasmid#106123PurposeIndicator for glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR
TagsMyc and PDGFRExpressionMammalianPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDR348 FANCF
Plasmid#47510Purposehuman gRNA expression vector targeting FANCFDepositorAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+MT-hToca-1-W518K
Plasmid#33031DepositorPublicationInsertToca-1 (FNBP1L Human)
Tags6xMycExpressionMammalianMutationW518KPromotersimian CMV IE94Available sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTE3327
Plasmid#107535PurposeExpresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.DepositorInsertsAs crRNA
hAsCpf1(RVR mutant)
Tags3xHA, NLS, and SV40 NLSExpressionMammalianMutationS542R, K548V, N552RPromoterCMV and human U6Available sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE3328
Plasmid#107539PurposeExpresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.DepositorInsertsAs crRNA
hAsCpf1(RR mutant)
Tags3xHA, NLS, and SV40 NLSExpressionMammalianMutationS542R, K607RPromoterCMV and human U6Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidExpressionMammalianPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only