We narrowed to 8,296 results for: construct

1. pSKB3SaGGR I206F (JBEI-3678)

   Plasmid

   #110149

   Purpose

   SaGGR site-directed mutation of I206F

   Depositor

   Jay Keasling

   Article

   Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

   Insert

   GGR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SaGGR site-directed mutation of I206F

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSKB3SaGGR G91H (JBEI-3677)

   Plasmid

   #110148

   Purpose

   SaGGR site-directed mutation of G91H

   Depositor

   Jay Keasling

   Article

   Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

   Insert

   GGR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SaGGR site-directed mutation of G91H

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSKB3SaGGR L377H (JBEI-3683)

   Plasmid

   #110150

   Purpose

   SaGGR site-directed mutation of L377H

   Depositor

   Jay Keasling

   Article

   Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

   Insert

   GGR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   SaGGR site-directed mutation of L377H

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTH861-gst-optec P210T

   Plasmid

   #89595

   Purpose

   Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed proline 210 to threonine

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTH846-gst-dissc

   Plasmid

   #96907

   Purpose

   Plasmid for yeast expression of GST encoded by non-preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   yeast codon dis-optimised glutathione-S transferase
   

   Use

   Tags

   8xhistidine

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTH860-gst-optec A180Q

   Plasmid

   #89594

   Purpose

   Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed alanine 180 to glutamine

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTH858-gst-optec V156E

   Plasmid

   #89592

   Purpose

   Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed valine 156 to glutamic acid

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTH859-gst-optec Y172M

   Plasmid

   #89593

   Purpose

   Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed tyrosine 172 to methionine

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E-GFP-HA no-pA

   Plasmid

   #82589

   Purpose

   3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   GFP-HA (no pA)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRPa-c6MYCn

    Plasmid

    #69242

    Purpose

    Tet-inducible RRNA promoter driven plasmid for expression of N or C-terminally c-Myc tagged protein from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    cMyc

    Expression

    Mutation

    Promoter

    RRNA promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRP-c6MYCn

    Plasmid

    #69243

    Purpose

    Tet-inducible RRNA promoter driven plasmid for inducible expression of N- or C-terminally c-Myc tagged protein from a random RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    cMyc

    Expression

    Mutation

    Promoter

    RRNA promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRPa-iSL(MCS3+4)

    Plasmid

    #69246

    Purpose

    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

    Depositor

    David Horn

    Article

    Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

    Type

    Empty backbone

    Use

    Expression in trypanosoma brucei

    Tags

    Expression

    Mutation

    Promoter

    RRNA promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHJ33

    Plasmid

    #48362

    Purpose

    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin selectable

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-loxP-3' ALD UTR
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDS69

    Plasmid

    #48367

    Purpose

    FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)

    Depositor

    George Cross

    Article

    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

    Insert

    FLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
    

    Use

    Cre/Lox; Knockout in t. brucei

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNH066

    Plasmid

    #42162

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    N-TAP Tag-RT-::mTFP1
    

    Use

    Tags

    TAP-TAg

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNH094

    Plasmid

    #42167

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1-RT-::[(G4S)3]::C-TAP Tag
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNH090

    Plasmid

    #42166

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1::[(G4S)3]::C-TAP Tag
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNH009

    Plasmid

    #42146

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    N-TAP-tag::mTFP1
    

    Use

    Tags

    TAP-Tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTAL13

    Plasmid

    #37186

    Depositor

    Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Type

    Empty backbone

    Use

    Tags

    FokI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHD8

    Plasmid

    #30981

    Depositor

    Daniel Voytas, Adam Bogdanove

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    repeat HD8
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge