We narrowed to 5,896 results for: dna plasmids

1. pCMV6-AN-DDK_C344A RAD23A

   Plasmid

   #201577

   Purpose

   Expresses a variant of human RAD23A containing mutation C344A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation C344A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_F354A RAD23A

   Plasmid

   #201578

   Purpose

   Expresses a variant of human RAD23A containing mutation F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation F354A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV6-AN-DDK_C344A_F354A RAD23A

   Plasmid

   #201579

   Purpose

   Expresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog A (RAD23A Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutations C344A and F354A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

   Plasmid

   #201545

   Purpose

   Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks N-terminal residues 1-79

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

   Plasmid

   #201546

   Purpose

   Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 188-228

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)

   Plasmid

   #201547

   Purpose

   Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks residues 276-339

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

   Plasmid

   #201548

   Purpose

   Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Lacks C-terminal residues 364-409

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DNMT2Δ191-237

   Plasmid

   #198382

   Purpose

   Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

   Depositor

   Tanja Schirmeister

   Article

   Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

   Insert

   DNMT2 (TRDMT1 Human)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 191-237

   Promoter

   T7

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HIS_TEV_hsSYX3

   Plasmid

   #179290

   Purpose

   E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Tags

   His6 + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7lac

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. hASXL(1-115)-luciferase

    Plasmid

    #106427

    Purpose

    Expresses human ASXL1 (amino acids 1-115) fused to the N-terminal of firefly luciferase

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    addition sex combs like 1 ASXL1 (ASXL1 Human)
    

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    amino acids 1-115 fused to luciferase

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AL62_pDonor.DMD.TS

    Plasmid

    #100287

    Purpose

    DMD intron 43-targeting plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    EGFP expression unit (DMD Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology; Human targeting

    Expression

    Mammalian

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. SL542

    Plasmid

    #49951

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-8 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

13. SL544

    Plasmid

    #49970

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-10 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. SL545

    Plasmid

    #49953

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-11 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

15. SL543

    Plasmid

    #49952

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-9 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. SL541

    Plasmid

    #49950

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-7 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

17. SL540

    Plasmid

    #49949

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD RH-6 (RHOXF2 Human)
    

    Use

    TALEN

    Promoter

    EF1a

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid

    #214688

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

    Depositor

    Kevin Janes

    Insert

    dgRNA_Chr1
    

    Use

    CRISPR and Lentiviral

    Mutation

    Puromycin resistance cassette has silent mutation…

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223 SARS-CoV-2 NSP5

    Plasmid

    #141259

    Purpose

    Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Has Service

    Cloning Grade DNA

    Insert

    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pMflT-o4

    Plasmid

    #101312

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4: rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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