We narrowed to 4,940 results for: puc

1. pR540

   Plasmid

   #207885

   Purpose

   template for 2 gRNA amplification

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   scaffold-pU6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS021

   Plasmid

   #207917

   Purpose

   Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKE013

   Plasmid

   #207916

   Purpose

   Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNC1136

   Plasmid

   #41562

   Depositor

   Beverly Errede

   Article

   Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

   Insert

   Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
   

   Use

   Tags

   Expression

   Mutation

   GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFPQ4

   Plasmid

   #83559

   Purpose

   Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL7 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL7

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLib6.6

   Plasmid

   #176652

   Purpose

   Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TC1092

   Plasmid

   #164879

   Purpose

   CMV-dcas13d-NLS-ADARdd- mCherry

   Depositor

   Tian Chi

   Article

   Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

   Insert

   dcas13d-ADARdd
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPotef-LN22*-3xGNLS-cbx

   Plasmid

   #86780

   Purpose

   Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

   Depositor

   Michael Feldbrügge

   Article

   Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

   Insert

   LN*
   

   Use

   Ustilago maydis expression

   Tags

   Gfp

   Expression

   Bacterial

   Mutation

   Promoter

   Potef

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE12

   Plasmid

   #79469

   Purpose

   sgRNA shuttle vector; module 5

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Insert

   pAtU6-ccdB-sgRNA (ccdB(letD) )
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI site eliminated

   Promoter

   Available since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE13

    Plasmid

    #79471

    Purpose

    sgRNA shuttle vector; module 6

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDGE14

    Plasmid

    #79473

    Purpose

    sgRNA shuttle vector; module 7

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGE15

    Plasmid

    #79474

    Purpose

    sgRNA shuttle vector; module 8E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDGE10

    Plasmid

    #79466

    Purpose

    sgRNA shuttle vector; module 4

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLib6.4B

    Plasmid

    #176665

    Purpose

    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAT15483_e-ABE8e

    Plasmid

    #174121

    Purpose

    Expresses ABE8e fused with eCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAT15001-AncBE4max

    Plasmid

    #163016

    Purpose

    plasmid expressing AncBE4max

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZH509

    Plasmid

    #102664

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIIS18-SapI

    Plasmid

    #128599

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIIS18-BsmBI

    Plasmid

    #128648

    Purpose

    Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFH51

    Plasmid

    #128213

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge