19,635 results

1. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEGFP-Lifeact-7

   Plasmid

   #54610

   Purpose

   Localization: Actin, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   mEGFP-Lifeact

   Expression

   Mammalian

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-CAG-RFP

   Plasmid

   #22910

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   Chicken Beta-actin promoter driving DsRedExpress
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Available Since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-25xUPRE-minP-EGFP

   Plasmid

   #159668

   Purpose

   EGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)

   Depositor

   Seiichi Oyadomari

   Article

   Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

   Insert

   25xUPRE-minP-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. At2S3:RUBY

   Plasmid

   #160906

   Purpose

   RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and At2S3 promoter
   

   Expression

   Plant

   Promoter

   At2S3

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF6-Acly(S455D)

   Plasmid

   #70768

   Purpose

   Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

   Depositor

   Kathryn Wellen

   Article

   Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

   Insert

   ATP Citrate Lyase (Acly Mouse)
   

   Tags

   myc-epitope

   Expression

   Mammalian

   Mutation

   S455D

   Promoter

   EF1alpha

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. FH-TET1-pEF

   Plasmid

   #49792

   Purpose

   This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

   Depositor

   Anjana Rao

   Article

   Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

   Insert

   Tet methylcytosine dioxygenase 1 (TET1 Human)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. hE-cadherin-pcDNA3

   Plasmid

   #45769

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin (CDH1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-tetO-myc T58A

   Plasmid

   #19763

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

   Insert

   c-myc (MYC Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T58A

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV enAsCas12f-HKRA-sgRNA_v7

    Plasmid

    #208357

    Purpose

    Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

    Depositor

    Atsushi Hoshino

    Article

    Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

    Insert

    enAsCas12f-HKRA
    

    Expression

    Mammalian

    Mutation

    I123H/D195K/D208R/V232A

    Promoter

    CMV

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDT305

    Plasmid

    #128801

    Purpose

    Human codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
    

    Use

    CRISPR and Lentiviral

    Promoter

    hUBC promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSOUP

    Plasmid

    #165419

    Purpose

    The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

    Insert

    Rep A
    

    Use

    Helper plasmid required for replication for all p…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL43.1 - Pglh-2::MosTase::glh-2utr

    Plasmid

    #19332

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Pglh-2::Mos1 transposase
    

    Expression

    Worm

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. VPS13A^mCherry

    Plasmid

    #118758

    Purpose

    VPS13A internally tagged with mCherry at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Tags

    mCherry inserted at position 1372

    Expression

    Mammalian

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHIV-EGFP

    Plasmid

    #21373

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

    Depositor

    Bryan Welm, Zena Werb

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. LPC-flag-REST-WT

    Plasmid

    #41903

    Depositor

    Stephen Elledge

    Article

    Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

    Insert

    RE1-silencing transcription factor (REST Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-BAIAP2 wt

    Plasmid

    #31675

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    BAI1-associated protein 2 (BAIAP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJRD215

    Plasmid

    #167555

    Purpose

    Backbone plasmid to pRMA154

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1994 Feb;176(3):733-47. doi

    Type

    Empty backbone

    Use

    Broad host range mobilisable

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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