We narrowed to 4,788 results for: AAT

1. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #173211

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV.U6gT28.3.EFS-NS.H2B-RFP

   Plasmid

   #170364

   Purpose

   Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Expressing nuclear RFP.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

   Insert

   H2B-RFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBtPIPLC_N168C His

   Plasmid

   #173809

   Purpose

   Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

   Depositor

   Anne Gershenson, Mary F Roberts

   Article

   He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

   Insert

   Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Asn168Cys for labeling

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pW188-lenti-spsgRNA-lacZ-pEF1s-NLS-mScarlet-I-P2A-BlastR

   Plasmid

   #170813

   Purpose

   Lentiviral vector to co-express a lacZ control spsgRNA with NLS-mScarlet-I

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Insert

   NLS-mScarlet-I-P2A-BlastR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pROS13_IDH2 #7

   Plasmid

   #166102

   Purpose

   This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH2 sgRNA #7 (IDH2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-sgCTRL-3

   Plasmid

   #83936

   Purpose

   Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCTRL-3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJC2-GPT1-3xFLAG

   Plasmid

   #163446

   Purpose

   lentiviral overexpression vector for human GPT1

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GPT1 (GPT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMKO.1-shNuf2

   Plasmid

   #160961

   Purpose

   Nuf2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nuf2 shRNA (Nuf2 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. px335-sgRNA-EF(Cas9n)-Npm1-R

   Plasmid

   #122331

   Purpose

   Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Npm1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. px335-sgRNA-EF(Cas9n)-Npm1-L

    Plasmid

    #122332

    Purpose

    Expresses sgRNA targeting mouse Npm1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Npm1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. px335-sgRNA-EF(Cas9n)-Dhx15-L

    Plasmid

    #122336

    Purpose

    Expresses sgRNA targeting mouse Dhx15 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ddx15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. px335-sgRNA-EF(Cas9n)-Birc6-R

    Plasmid

    #122338

    Purpose

    Expresses sgRNA targeting mouse Birc6 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Birc6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. px335-sgRNA-EF(Cas9n)-Trim27-L

    Plasmid

    #122317

    Purpose

    Expresses sgRNA targeting mouse Trim27 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Trim27
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. px335-sgRNA-EF(Cas9n)-Rad21-L

    Plasmid

    #122313

    Purpose

    Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Rad21
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. px335-sgRNA-EF(Cas9n)-Ssrp1-L

    Plasmid

    #122315

    Purpose

    Expresses sgRNA targeting mouse Ssrp1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Ssrp1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA0057

    Plasmid

    #131784

    Purpose

    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer GFP cassette guide (gGFP) and 5' sgRNA
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgMLST8_4

    Plasmid

    #124908

    Purpose

    Targeting human MLST8 gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

    Plasmid

    #121509

    Purpose

    Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah.intron9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. gh85

    Plasmid

    #106745

    Purpose

    expression of gRNA targeting FUT1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    FUT1 (FUT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNAs[bTub+Tra].1046D

    Plasmid

    #112693

    Purpose

    express two gRNA targeting bTub & Tra under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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