159,428 results

1. 2xP53_RE::FLuc

   Plasmid

   #178316

   Purpose

   P53 Firefly luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Insert

   P53 FLuc Reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2xmyc-JIP4

   Plasmid

   #164633

   Purpose

   Expresses JIP4 in mammalian cells with a 2xmyc tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   JIP4 (SPAG9 Human)
   

   Use

   Tags

   2xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVSVΔG-eGFP-linker

   Plasmid

   #231707

   Purpose

   Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV antigenome
   

   Use

   Vsv expression

   Tags

   Expression

   Mammalian

   Mutation

   ΔG (VSV glycoprotein removed)

   Promoter

   T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-neo-cterminal-3HA

   Plasmid

   #102643

   Purpose

   To generate three copies of the HA epitope tag on cDNAs.

   Depositor

   Michael E. Wright

   Type

   Empty backbone

   Use

   Tags

   3x HA (YPYDVPDYA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ecoli PancACe

   Plasmid

   #215712

   Purpose

   Expresses acetyl-CoA biosensor in Ecoli

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-EFS-EGFP-T2A-Bsd

   Plasmid

   #193198

   Purpose

   Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Use

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mRFP-Rab7

   Plasmid

   #14436

   Purpose

   Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 7 (RAB7A Canis familialis)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   3 silent mutations near the C-terminus

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

    Plasmid

    #175508

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP_mNG2(11)_Clathrin light chain

    Plasmid

    #82608

    Purpose

    Expresses mNG2(11) tagged clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNG2(11)_Clathrin light chain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-CalfluxVTN

    Plasmid

    #83926

    Purpose

    Bioluminescent Reporter of Intracellular Calcium

    Depositor

    Carl Johnson

    Article

    Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

    Insert

    CalfluxVTN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMB80 (R26-CreER)

    Plasmid

    #12168

    Depositor

    Tyler Jacks

    Article

    McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

    Insert

    Rosa26 CreER^T2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-BRCA1-mCherry

    Plasmid

    #195275

    Purpose

    express mCherry-BRCA1 fusion

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    mCherry-BRCA1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-Q74

    Plasmid

    #40262

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-Hylight

    Plasmid

    #193447

    Purpose

    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

    Depositor

    Richard Goodman

    Article

    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Hylight Biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. hBax C3-EGFP

    Plasmid

    #19741

    Depositor

    Richard Youle

    Article

    Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

    Insert

    Bax (BAX Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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