161,873 results

1. pMRX-IP-GFP-LC3-RFP

   Plasmid

   #84573

   Purpose

   Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. V5-GFP-TurboID-KDEL_pLX208

   Plasmid

   #202020

   Purpose

   expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   V5-TurboID-KDEL
   

   Use

   Lentiviral

   Tags

   GFP and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUGW-hGtACR2-EYFP

   Plasmid

   #67877

   Purpose

   Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

   Depositor

   John Spudich

   Article

   Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

   Insert

   hGtACR2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE-Tight-MitoTimer

   Plasmid

   #50547

   Purpose

   Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

   Depositor

   Roberta Gottlieb

   Article

   Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

   Insert

   MitoTimer
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F12S mutation in Timer

   Promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRUPATH Triple Ga15

   Plasmid

   #196060

   Purpose

   Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha15-RLuc8 (GNA15 Human)
   GGamma13-GFP2 (GNG13 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRVL-4

   Plasmid

   #104582

   Purpose

   Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Human CL kappa
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-FLAG-SF3B1-WT

   Plasmid

   #82576

   Purpose

   Expresses a codon-optimized ORF of human SF3B1 (wild-type)

   Depositor

   Manoj Pillai

   Article

   Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

   Insert

   human SF3B1-WT (SF3B1 Human)
   

   Use

   Tags

   FLAG peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CXCR4

   Plasmid

   #98942

   Purpose

   Mammalian expression plasmid for untagged human CXCR4

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH3.29_Cre

   Plasmid

   #205560

   Purpose

   Express Cre (without export tag)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST26 Ago2

    Plasmid

    #59904

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR223-EBNA2

    Plasmid

    #195815

    Purpose

    Gateway Entry Clone of Epstein-Barr virus EBNA2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA2 (EBNA-2 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Mariner2_AG

    Plasmid

    #217135

    Purpose

    Mammalian expression vector for expression of Mariner2_AG transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    Mariner2_AG transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRVL-2

    Plasmid

    #104580

    Purpose

    Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Mouse IgG2c CH1/CH2/CH3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymScarletI3-URA3

    Plasmid

    #224075

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection

    Depositor

    Bas Teusink

    Insert

    ymScarletI3
    

    Use

    Tags

    ymScarletI3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-PDGFRB

    Plasmid

    #23893

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188779

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4-p100

    Plasmid

    #23287

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    NFkB p100 subunit (NFKB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-EF1alpha-ZsGreen1_Puro

    Plasmid

    #192661

    Purpose

    3rd generation lenti vector encoding ZsGreen1 with 2A Puro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    ZsGreen1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T MDM2 WT

    Plasmid

    #16237

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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