We narrowed to 4,337 results for: 1-Oct;

1. DR274-eGFP sgRNA

   Plasmid

   #61051

   Purpose

   for generation of a eGFP specific sgRNA from a T7 promoter

   Depositor

   Filippo Del Bene

   Article

   Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

   Insert

   EGFP sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIntegrase_9

   Plasmid

   #60580

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCis_2+7+8+5

   Plasmid

   #60589

   Purpose

   a 4-integrase plasmid

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cis_2+7+8+5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas_2+5

   Plasmid

   #60594

   Purpose

   encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_2+5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK5-mWnt1

   Plasmid

   #42273

   Depositor

   Chris Garcia, Jeremy Nathans

   Article

   Yu et al Development. 2012 Dec 1;139(23):4383-94.

   Insert

   Wnt1 (Wnt1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pY1

   Plasmid

   #50597

   Purpose

   Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

   Depositor

   Jay Keasling

   Article

   Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

   Type

   Empty backbone

   Use

   Synthetic Biology; Empty vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLacUV5

   Available since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCis_7+8+10

   Plasmid

   #60588

   Purpose

   a 3-integrase plasmid

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cis_7+8+10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GB4653

   Plasmid

   #215267

   Purpose

   TU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoter

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   pNos:FLP:tNos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GB4650

   Plasmid

   #215265

   Purpose

   TU for the copper regulated expression of the PhiC31 recombinase.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS:miniDFR:PhiC31:tNos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GB4832

    Plasmid

    #215276

    Purpose

    Module for the constitutive expression of HISPS, H3H, EGFP and p23.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    HISPS-H3H-EGFP-p23
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB4652

    Plasmid

    #215266

    Purpose

    TU for the copper regulated expression of the flippase (FLP) recombinase.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS:miniDFR:FLP:TNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GB4666

    Plasmid

    #215271

    Purpose

    pUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 promoter.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    promMAPKKK18
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GB1203

    Plasmid

    #215369

    Purpose

    TU for the expression of the silencing suppressor P19 driven by the 35s promoter

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    35s:P19:tNos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLT237

    Plasmid

    #174300

    Purpose

    Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    PolC homology arm
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Cysteine 669 to Tyrosine

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGO186N-KS2

    Plasmid

    #174302

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB-puro-mU6-RtcAshRNA1

    Plasmid

    #126613

    Purpose

    Expresses shRNA against human RtcA under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    RtcA shRNA-1
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFL-SV40-PACT 5'-UTR

    Plasmid

    #115351

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of PACT

    Depositor

    Ming-Chih Lai

    Article

    Lai et al FEBS J. 2016 Jan;283(1):88-101. doi: 10.

    Insert

    PACT 5'-UTR (PRKRA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUW-SOKM

    Plasmid

    #20325

    Purpose

    Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastbac-Flag-BRCA1

    Plasmid

    #223228

    Purpose

    Insect expression construct for Flag-tagged BRCA1 protein production and purification

    Depositor

    Weixing Zhao

    Article

    Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

    Insert

    Breast Cancer 1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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