Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,197 results for: 338

Showing: 3461 - 3480 of 6197 results

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LENTICRISPR-PFKM_sgRNA2

Plasmid

#201611

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PFKM (PFKM Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N__C192A

Plasmid

#201176

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_C192A

Plasmid

#201172

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C192A

Promoter

TRE3G

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_C39A

Plasmid

#201175

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C39A

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_C39A

Plasmid

#201171

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C39A

Promoter

TRE3G

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Mutation

Cas4 domain (K81A)

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1642-GB1His6_AH

Plasmid

#148890

Purpose

Bacterial Expression of HsHelz_1475-1642

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1642 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1475-1942-GB1His6_AH

Plasmid

#148891

Purpose

Bacterial Expression of HsHelz_1475-1942

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1475-1942 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-HsHelz_1643-1792-GB1His6_AH

Plasmid

#148893

Purpose

Bacterial Expression of HsHelz_1643-1792

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1643-1792 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsHelz_1793-1914-GB1His6_AH

Plasmid

#148894

Purpose

Bacterial Expression of HsHelz_1793-1914

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1914 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsHELZ_1793-1942-GB1His6_AH

Plasmid

#148896

Purpose

Bacterial Expression of HsHelz_1793-1942

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1793-1942 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-2STREP-HsHelz-GSSG-MBP_AG

Plasmid

#148796

Purpose

Mammalian Expression of HsHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz (HELZ Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnEA-NvM-HsHelz_1051-1474-GB1His6_AG

Plasmid

#148798

Purpose

Bacterial Expression of HsHelz_1051-1474

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz_1051-1474 (HELZ Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSUPERpuro-BglII-HsHelz_AC

Plasmid

#148474

Purpose

Mammalian Expression of HsHelz-shRNA

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz-shRNA (HELZ Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1_4-302_S

Plasmid

#147536

Purpose

Mammalian Expression of HsNot1iso1_4-302

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_4-302 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1_1356-2370_S

Plasmid

#147539

Purpose

Mammalian Expression of HsNot1iso1_1356-2370

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1356-2370 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1147-1717-K1277A_R

Plasmid

#147431

Purpose

Insect Expression of DmNot1_1147-1717-K1277A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-1717-K1277A (Not1 Fly)

Expression

Insect

Mutation

2 non silent mutations compared to the sequence g…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1149-2505-dsRNAres_R

Plasmid

#147432

Purpose

Insect Expression of DmNot1_1149-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1149-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Showing: 3461 - 3480 of 6197 results

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