We narrowed to 5,560 results for: Chia

1. pdCas9-M-1B6

   Plasmid

   #73430

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1B6.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1B6 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-M-3H5

   Plasmid

   #73429

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3H5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 3H5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-M-1D4

   Plasmid

   #73433

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1D4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1D4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdCas9-M-1E4

   Plasmid

   #73436

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 1E4.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 1E4 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9-M-5F5

   Plasmid

   #73432

   Purpose

   CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Repressor 5F5 (orthogonal T7-lac repressor)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive wild-type S. pyogenes promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

   Plasmid

   #202762

   Purpose

   Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   NEUROG2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

   Plasmid

   #73440

   Purpose

   Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC-P3A2-vioD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV[Exp]-EF1A>Tet3G:IRES:Neo

   Plasmid

   #198756

   Purpose

   Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Tet3G, IRES, Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1A

   Available since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFP.E222

   Plasmid

   #138248

   Purpose

   Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   ECF20.N.(VDA).M86.N2
   Terminator
   SspGyrB.C2.(SAK).ECF16.C
   Terminator
   rhaS
   ECF17.N.(NPC).NrdJ-1.N2
   Terminator
   M86.C2.(SDL).ECF20.C
   Terminator
   luxR
   Terminator
   ECF16.N.(AGG).SspGyrB.N2
   Terminator
   NrdJ-1.C2.(SEI).ECF17.C
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZE21/UBP1/ClpS_V65I

    Plasmid

    #98567

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    Mutated ATP-dependent Clp protease adapter protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains the V65I mutation that increases discrim…

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTO_CHMP3_LAP

    Plasmid

    #101849

    Purpose

    Tet-inducible expression of human CHMP3 with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    Charged Multivesicular Body Protein 3 (CHMP3 Human)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCC1-4k-BBclone2

    Plasmid

    #137070

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0323
    P13
    DipA
    Lmp1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-YFP-KRAS4B

    Plasmid

    #112718

    Purpose

    Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    YFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CFP-KRAS4B

    Plasmid

    #112717

    Purpose

    Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    CFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLY164

    Plasmid

    #184140

    Purpose

    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-R3, PrhaB, and Ptet

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLY305

    Plasmid

    #184159

    Purpose

    Reporter circuit for hijacking mRNA of zraP as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    mutsfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    K140N, Mutations D10A and H840A on WT cas9. Synon…

    Promoter

    Pcon, PpspA-zraP-22, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAJ1

    Plasmid

    #138379

    Purpose

    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ

    Depositor

    Seth Darst

    Article

    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11

    Inserts

    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…

    Promoter

    T7

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

    Plasmid

    #240865

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

    Plasmid

    #240094

    Purpose

    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes

    Depositor

    Mark Verheijen

    Article

    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23

    Insert

    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    

    Use

    AAV and CRISPR

    Tags

    SaCas9 + 3X HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. P527_POU4F2-p2A-mNeonGreen-h2b

    Plasmid

    #239103

    Purpose

    Azenta NGS; inserts a p2A-mNeonGreen-h2b fusion at the endogenous POU4F2 (aka BRN3B) gene

    Depositor

    Karl Wahlin

    Article

    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Tags

    p2A-mNeonGreen-h2b

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous POU4F2

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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