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pRepair-mTq2-CTNNB1

Plasmid

#153433

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mTurquoise2

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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SS3 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145821

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS3 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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SS2 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145820

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS2 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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SS32 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145822

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS32 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-ZNF423 [N328B/37R]

Plasmid

#140077

Purpose

Mammalian Expression Plasmid of anti-ZNF423 (Human). Derived from hybridoma N328B/37.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-ZNF423 (Human) recombinant mouse monoclonal antibody (ZNF423 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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SS44 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145824

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS44 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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SS88 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145825

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS88 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [dRING]

Plasmid

#126549

Purpose

Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RING domain deletion mutant [dRING mutation is a …

Promoter

LTR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [Y252D]

Plasmid

#126550

Purpose

Expresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Y252D point mutant

Promoter

LTR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-mRFP

Plasmid

#117787

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Bod1l_Anti-Sense

Plasmid

#124444

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

Biorientation of chromosomes in cell division 1-like (Bod1l Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Gli3_Sense

Plasmid

#124439

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

GLI-Kruppel family member GLI3 (Gli3 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-REEP1/2 [N326D/29R]

Plasmid

#114510

Purpose

Mammalian Expression Plasmid of anti-REEP1/2 (Mouse). Derived from hybridoma N326D/29.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-REEP1/2 (Mus musculus) recombinant mouse monoclonal antibody (Reep1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-PARIS/ZNF746 [N196/16R]

Plasmid

#114475

Purpose

Mammalian Expression Plasmid of anti-PARIS/ZNF746 (Human). Derived from hybridoma N196/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PARIS/ZNF746 (Homo sapiens) recombinant mouse monoclonal antibody (ZNF746 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Malin [N85/18.1R]

Plasmid

#114513

Purpose

Mammalian Expression Plasmid of anti-Malin (Human). Derived from hybridoma N85/18.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Malin (Homo sapiens) recombinant mouse monoclonal antibody (NHLRC1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Stonin-2 [N346/9R]

Plasmid

#114484

Purpose

Mammalian Expression Plasmid of anti-Stonin-2 (Human). Derived from hybridoma N346/9.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Stonin-2 (Homo sapiens) recombinant mouse monoclonal antibody (STON2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL c2x Tral FL-15D

Plasmid

#113010

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to D

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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GIV-FLAG L249P (LP)

Plasmid

#86097

Purpose

Expression GIV in mammalian cell

Depositor

Pradipta Ghosh

Article

Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

Insert

GIV/Girdin (CCDC88A Human)

Tags

3xFlag

Expression

Mammalian

Mutation

L249P

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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Plxnb2(S)-AP-His

Plasmid

#72003

Purpose

Expresses the N-terminal extracellular region of the PlexinB2 protein following proteolytic cleavage (ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb2 (Plxnb2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Jam4.3-Fc-His

Plasmid

#72082

Purpose

Expresses the extracellular region of the JAM-4, isoform 3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam4.3 (Igsf5 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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