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We narrowed to 66,706 results for: nin

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Showing: 3461 - 3480 of 66706 results

  1. hCMV-IE1:Renilla

    Plasmid
    #118066
    Purpose
    Transcriptional unit for constitutive expression of Renilla in mammalian cells
    Depositor
    Koen Venken
    Publication
    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV:GreenRenilla:bGHF
    Use
    Luciferase and Synthetic Biology; Assembly of gb2…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118066-plasmid-map-sequence-id-240497

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  2. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  8. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80424-plasmid-map-sequence-id-151441

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  9. pTXB1-sfGFP

    Plasmid
    #51562
    Purpose
    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    superfolder GFP
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51562-plasmid-map-sequence-id-78800

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  16. PB-PEmax

    Plasmid
    #187893
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187893-plasmid-map-sequence-id-370734

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  18. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140973-plasmid-map-sequence-id-300655

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Showing: 3461 - 3480 of 66706 results