We narrowed to 9,393 results for: Coli

1. GST_LIM234

   Plasmid

   #182039

   Purpose

   For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

   Depositor

   Vesa Hytönen

   Article

   Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

   Insert

   LIM234 (PXN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   LIM domains 2-4 only

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pThuAsol

   Plasmid

   #171675

   Purpose

   pThuAsol expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   SUMO-TEV-ThuAsol
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLyGo-Ec-4

   Plasmid

   #163130

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLyGo-Ec-1

   Plasmid

   #163127

   Purpose

   pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Ubiquitin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLyGo-Ec-3

   Plasmid

   #163129

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLyGo-Ec-2

   Plasmid

   #163128

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLyGo-Ec-5

   Plasmid

   #163131

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSF-ChPylTMSK

   Plasmid

   #163915

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Abdelkader et al J Am Chem Soc. 2021 Jan 5. doi: 10.1021/

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX6P1-optimizedNedd4.2F823D

   Plasmid

   #165105

   Purpose

   bacterial expression of a GST fusion of Human Nedd4.2d F823D

   Depositor

   Arthur Haas

   Article

   Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

   Insert

   Nedd4.2d F823D (NEDD4L Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression; F823D; …

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

    Plasmid

    #153443

    Purpose

    E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.XDocB
    

    Use

    Tags

    6xHis and ybbr tag

    Expression

    Bacterial

    Mutation

    Serine 199 was mutated to amber codon (TAG)

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB1C3_mTq-Tat (c-plasmid)

    Plasmid

    #127635

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: mTurquoise2-tat.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme PlmJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PCP and RNA binding peptide:…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCool-human TSC1 (939-992)

    Plasmid

    #132618

    Purpose

    expresses human TSC1 (939-992) in E. coli

    Depositor

    Wenqing Xu

    Article

    Qin et al J Biol Chem. 2016 Apr 15;291(16):8591-60

    Insert

    TSC1 (939-992)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEAST_J23101-bphD

    Plasmid

    #128138

    Purpose

    The cell-free adapted backbone, pBEAST, expressing bphD (the enzyme converting 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    bphD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC0.066

    Plasmid

    #119666

    Purpose

    Level 0 part. Terminator from pheA gene from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    TpheA-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC0.074

    Plasmid

    #119663

    Purpose

    Level 0 part. Terminator from Cysteine synthase A gene (cysK) from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120048902
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC0.071

    Plasmid

    #119662

    Purpose

    Level 0 part. Terminator from tatABCD operon from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120030798
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC0.069

    Plasmid

    #119661

    Purpose

    Level 0 part. Terminator from arcA gene from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120026481
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0.073

    Plasmid

    #119660

    Purpose

    Level 0 part. Terminator from rplJL-rpoBC,rplKAJL-rpoBC,rpoBC gene cluster from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120010869
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC0.065

    Plasmid

    #119659

    Purpose

    Level 0 part. Terminator araBAD operon from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120010860
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC0.068

    Plasmid

    #119658

    Purpose

    Level 0 part. Terminator for clpPX gene from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120010850
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pC0.072

    Plasmid

    #119657

    Purpose

    Level 0 part. Terminator from hyaABCDEF operon from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120010842
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pC0.077

    Plasmid

    #119656

    Purpose

    Level 0 part. Terminator from rpoH gene from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120010841-R
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pC0.064

    Plasmid

    #119655

    Purpose

    Level 0 part. Terminator from hupB gene from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    ECK120010820
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC0.070

    Plasmid

    #119652

    Purpose

    Level 0 part. Terminator, Bi-directional terminator for yciA/tonA genes from E. coli.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    BBa_B0061
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMH-HT_CtCHAD-H253A-R256A-R260A

    Plasmid

    #127788

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMH-HT_CtCHAD-H253A-R256A-R260A-R296A

    Plasmid

    #127789

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in His2…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMH-HT_CtCHAD-R385A-K386A-K389A-K390A

    Plasmid

    #127792

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg3…

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET27b-ecDHFR-N23PP

    Plasmid

    #110828

    Purpose

    Bacterial expression of E.coli DHFR N23PP mutant

    Depositor

    Stephen Benkovic

    Article

    Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23; a proline residue inserted be…

    Promoter

    T7

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28b+3(R112C/T180C)C

    Plasmid

    #108193

    Purpose

    Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA3(R112C/T180C)-P450cam fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET15b+2(L171C)X

    Plasmid

    #108192

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA2(L171C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET15b+R2(L171C)X

    Plasmid

    #108191

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET15b+C3(R112C/T180C)X

    Plasmid

    #108196

    Purpose

    Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    P450cam-PCNA3(R112C/T180C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET15b+1(G108C)X

    Plasmid

    #108189

    Purpose

    Expresses PCNA1(G108C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1(G108C)-PdX fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C (PCNA1); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD-Tam1

    Plasmid

    #101639

    Purpose

    Encodes the fluorescent protein Tam1 for arabinose-inducible expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    Tam1
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pThom77.3

    Plasmid

    #101636

    Purpose

    Encodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    amFP486/K68M
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    K68M

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET21a - MPX

    Plasmid

    #89619

    Purpose

    [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Mitocondrial Tryparedoxin peroxidase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET21a - EtfB

    Plasmid

    #89617

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    electron-transfer-flavoprotein, alpha polypeptide
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET21a - TDR

    Plasmid

    #89627

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    thiol dependent reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge