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We narrowed to 8,877 results for: BLI;

Showing: 8751 - 8800 of 8877 results

  1. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  4. pCIBN-tagBFP-hTRF2

    Plasmid
    #103803
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103803-plasmid-map-sequence-id-199226

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  8. pCIBN-EYFP-hTRF2

    Plasmid
    #103804
    Purpose
    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-EYFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103804-plasmid-map-sequence-id-199232

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  9. pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

    Plasmid
    #221081
    Purpose
    SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication
    Depositor
    Mikhail Shapiro
    Article
    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.
    Insert
    ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221081-plasmid-map-sequence-id-438468

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  13. Human b4 nicotinic receptor subunit EM construct

    Plasmid
    #163961
    Purpose
    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    StrepII tag and apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Pro341-Ser395 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163961-plasmid-map-sequence-id-323883

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  17. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Use
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

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  18. pPHR-mCherry-NLS-VP16

    Plasmid
    #103821
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    PHR-mCherry-VP16 (CRY2 Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    103821-plasmid-map-sequence-id-203431

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  22. pPHR-iRFP713-NLS-VP16

    Plasmid
    #103823
    Purpose
    Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    PHR-iRFP713-VP16 (CRY2 Mustard Weed)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    VP16: G126T (silent, G42G)
    Promoter
    CMV
    Available since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    103823-plasmid-map-sequence-id-199372

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  23. DSPQ-HTT-TALEN2 BSD

    Plasmid
    #92246
    Purpose
    TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN
    Depositor
    Mahmoud Pouladi
    Article
    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7
    Insert
    TALEN
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    92246-plasmid-map-sequence-id-197973

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  24. DSPQ-HTT-TALEN1 ZEO

    Plasmid
    #92245
    Purpose
    TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD
    Depositor
    Mahmoud Pouladi
    Article
    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7
    Insert
    TALEN
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    92245-plasmid-map-sequence-id-197977

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  25. LIC-Z-YFP

    Plasmid
    #153544
    Purpose
    perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication
    Depositor
    Katharina Gaus
    Article
    Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:
    Insert
    human TCR ζ-Chain (CD247 Human)
    Use
    Tags
    Venus YFP
    Expression
    Mammalian
    Mutation
    with mCherry in LIC-Z replaced by YFP
    Promoter
    CMV
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    153544-plasmid-map-sequence-id-305908

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  33. 6xHsa.NFKB:Kaede

    Plasmid
    #239992
    Purpose
    Kaede driven by NFkB activity can be switched from green to red fluorescence by UV light exposure, enabling tracking of NFkB positive cells
    Depositor
    Raquel Espin-Palazon
    Insert
    photo convertible Kaede
    Use
    Tags
    NA
    Expression
    Mutation
    Promoter
    cfos minimal promoter
    Available since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    239992-plasmid-map-sequence-id-477299

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  35. pSCrhaB2_ApyD_ RBS_ApyAOHIS

    Plasmid
    #226247
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Use
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226247-plasmid-map-sequence-id-450057

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  36. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

    Plasmid
    #224505
    Purpose
    Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    LexA-mODC-LexOP-35SCaMV
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    224505-plasmid-map-sequence-id-447028

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  40. pCMV(MinDis)-iCyt_BrEt_SnFR_PM

    Plasmid
    #177747
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177747-plasmid-map-sequence-id-349063

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  41. _pCMV(MinDis)-iCyt_F_SnFR_PM

    Plasmid
    #177745
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177745-plasmid-map-sequence-id-349553

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  43. pCMV(MinDis)-iCyt_BrEt_SnFR_ER

    Plasmid
    #177746
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177746-plasmid-map-sequence-id-349555

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  44. _pCMV(MinDis)-iCyt_F_SnFR_ER

    Plasmid
    #177744
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177744-plasmid-map-sequence-id-349548

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  49. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid
    #55613
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    Use
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    Silent mutations have been incorporated into the …
    Promoter
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55613-plasmid-map-sequence-id-86499

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  50. pOGS539_haPD-1

    Plasmid
    #226716
    Purpose
    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)
    Depositor
    Gautam Dantas
    Article
    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2
    Insert
    High affinity PD-1 microbody
    Use
    Tags
    HA tag and Mating factor alpha secretion signal
    Expression
    Yeast
    Mutation
    Promoter
    pTEF1
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    226716-plasmid-map-sequence-id-455452

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Showing: 8751 - 8800 of 8877 results