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Showing: 8751 - 8800 of 167150 results

  1. mApple-Peroxisomes-2

    Plasmid
    #54627
    Purpose
    Localization: Peroxisomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    mApple-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. R777-E159 Hs.PDPK1

    Plasmid
    #70443
    Purpose
    Gateway ORF clone of human PDPK1 [NM_002613.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PDPK1 (PDPK1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70443-plasmid-map-sequence-id-125892

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  5. FUW-hTie1-FLAG

    Plasmid
    #119233
    Purpose
    Plasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    TIE1 (TIE1 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Promoter
    P hUbC
    Available Since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    119233-plasmid-map-sequence-id-231732

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  6. mPlum-Peroxisomes-2

    Plasmid
    #54509
    Purpose
    Localization: Peroxisomes, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    mPlum-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pDUAL OCLN (GFP)

    Plasmid
    #86982
    Purpose
    Lentiviral expression construct encoding Occludin and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    OCLN (OCLN Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV/hPGK
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86982-plasmid-map-sequence-id-173293

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  8. pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby

    Plasmid
    #205280
    Purpose
    Synaptic activity can be imaged with Synaptophysin GCaMP, while simultaneously, a red fluorescence structural marker can be employed to provide a visual reference for the cellular architecture.
    Depositor
    Matthias Prigge
    Insert
    GCaMP8s
    Use
    AAV
    Available Since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205280-plasmid-map-sequence-id-412569

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  10. PP-GSK3

    Plasmid
    #25133
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd4_240:C29838
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pRV-TdTom-MCS

    Plasmid
    #193711
    Purpose
    Retroviral plasmid DNA for subcloning an oligonucleotide library encoding RNA barcodes
    Depositor
    Colin Akerman
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    193711-plasmid-map-sequence-id-387442

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  12. pLV-EF1-FLP-PGK-Neo

    Plasmid
    #108544
    Purpose
    Lentivirus vector expessing Flp under EF1a promoter and neomycin under PGK promoter
    Depositor
    Javier Alcudia
    Inserts
    flp recombinase
    Neomycin
    Promoter
    EF1apha and PGK
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108544-plasmid-map-sequence-id-208673

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  15. pFastBacMam8_N_Flag_3C

    Plasmid
    #178818
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins.
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    Flag, 3C
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178818-plasmid-map-sequence-id-351926

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  18. pQCasTns(Ptr)-entry(BsaI)

    Plasmid
    #190274
    Purpose
    Expression of CRISPR-associated transposases
    Depositor
    Sheng Yang
    Insert
    PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(BsaI)
    Expression
    Bacterial
    Mutation
    no
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    190274-plasmid-map-sequence-id-403364

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  19. TC9pAB

    Plasmid
    #71310
    Purpose
    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9
    Use
    In vitro transcription
    Tags
    NLS
    Promoter
    T7
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71310-plasmid-map-sequence-id-129410

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  20. pAAV_Ef1a_DIO_MyrPalm_mScarlet_WT_IRES_mNeon_minBack

    Plasmid
    #205282
    Purpose
    In order to efficiently visualize projection and cell somata in light-sheet preparations, a combination of membrane-targeted and diffusible red fluorophores was developed. Additionally an ALFA tag
    Depositor
    Matthias Prigge
    Insert
    mScarlet
    Use
    AAV
    Available Since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205282-plasmid-map-sequence-id-412559

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  22. HT147_pAAV_EF1a_fDiO_somCheRiff_CFP

    Plasmid
    #208608
    Purpose
    Expressing soma-targeted CheRiff_CFP in a Flp-dependent manner
    Depositor
    Adam Cohen
    Insert
    soma-targeted CheRiff_CFP
    Use
    AAV
    Tags
    CFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208608-plasmid-map-sequence-id-414423

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  23. PP-G6PI

    Plasmid
    #28283
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-G6PI (PF14_0341 Plasmodium falciparum)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    28283-plasmid-map-sequence-id-235428

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  24. pLenti-CMV-GFP-miRE_shSCR-Puro

    Plasmid
    #135689
    Purpose
    Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shScrambled
    Use
    Lentiviral
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135689-plasmid-map-sequence-id-327714

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  25. pFastBac1/MBP-His

    Plasmid
    #38008
    Depositor
    Michael J Fox Foundation MJFF
    Insert
    MBP (Maltose binding protein)
    Use
    Baculovirus expression
    Tags
    6xHIS
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    Oct. 1, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pDGB3 alpha1 P35S:BFP:T35S (GB3416)

    Plasmid
    #160637
    Purpose
    TU for the constitutive expression of Blue Fluorescent Protein
    Depositor
    Diego Orzaez
    Insert
    BFP
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160637-plasmid-map-sequence-id-321961

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  27. mPA-GFP-Lifeact-7

    Plasmid
    #54671
    Purpose
    Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Lifeact-mPA-GFP
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. AAV-syn-axon-jGCaMP8m-WPRE

    Plasmid
    #186042
    Purpose
    jGCaMP8m expression in axons under the control of the synapsin promoter
    Depositor
    Loren Looger
    Insert
    axon-jGCaMP8m
    Use
    AAV
    Tags
    GAP43-6xHis
    Expression
    Mammalian
    Promoter
    synapsin
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186042-plasmid-map-sequence-id-370162

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  30. tdEos-MCPA2-C-18

    Plasmid
    #57639
    Purpose
    Localization: Actin Capping Protein , Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    MCPA2
    Tags
    tdEos
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57639-plasmid-map-sequence-id-468023

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  31. pBS mouse IRS-1

    Plasmid
    #11026
    Depositor
    Ronald Kahn
    Insert
    IRS-1 (Irs1 Mouse)
    Expression
    Bacterial
    Available Since
    Aug. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  33. pDSP32

    Plasmid
    #199374
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199374-plasmid-map-sequence-id-390801

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  34. TP2_crtYB

    Plasmid
    #140233
    Purpose
    Expression of gene coding lycopene bifunctional β-cyclase for production beta-carotene from lycopene
    Depositor
    Kang Zhou
    Insert
    phytoene-beta carotene synthase
    Expression
    Bacterial
    Mutation
    na
    Promoter
    t7 promoter
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    140233-plasmid-map-sequence-id-288147

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  35. R777-E222 Hs.RALGDS-nostop

    Plasmid
    #70506
    Purpose
    Gateway ORF clone of human RALGDS [NM_006266.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RALGDS (RALGDS Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70506-plasmid-map-sequence-id-125955

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  36. mApple-Talin-N-10

    Plasmid
    #54951
    Purpose
    Localization: Focal Adhesions, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Talin
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    H2541R in Talin (last residue before linker)
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pCDH-EF1-Fon-tdTomato

    Plasmid
    #72261
    Purpose
    Express tdTomato when FLP is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    elongation factor-1
    Available Since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72261-plasmid-map-sequence-id-131822

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  40. pCAGGS-ROSAf

    Plasmid
    #54323
    Purpose
    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid
    Depositor
    Silvestro Conticello
    Insert
    TALEN for the murine ROSA26 locus
    Use
    Mouse Targeting and TALEN
    Expression
    Mammalian
    Promoter
    CAGG
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    54323-plasmid-map-sequence-id-124428

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  41. tdTomato-MyosinIIB-N-18

    Plasmid
    #58119
    Purpose
    Localization: Cytoskeleton, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    58119-plasmid-map-sequence-id-226642

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  42. mVenus-Peroxisomes-2

    Plasmid
    #54692
    Purpose
    Localization: Peroxisomes, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    mVenus-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  43. pET-His6-Sumo-MBP-CfaN

    Plasmid
    #126016
    Purpose
    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)
    Depositor
    Thomas Muir
    Insert
    His5-Sumo-MBP-CfaN
    Tags
    5xHis-SUMO
    Expression
    Bacterial
    Available Since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126016-plasmid-map-sequence-id-248770

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  45. pLenti6.3_HES4

    Plasmid
    #124072
    Purpose
    HES4 with normal stop codon
    Depositor
    J. Chad Brenner
    Insert
    HES4 (HES4 Human)
    Use
    Lentiviral
    Available Since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124072-plasmid-map-sequence-id-241438

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  46. R777-E279 Hs.RHOB

    Plasmid
    #70563
    Purpose
    Gateway ORF clone of human RHOB [NM_004040.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RHOB (RHOB Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70563-plasmid-map-sequence-id-126012

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  47. mVenus-UbiquitinC-C-18

    Plasmid
    #56393
    Purpose
    Localization: Membrane, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    UbiquitinC (UBA52 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    aa 2-76 of NP_003324.1
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. pPICZa-STE2-1D4

    Plasmid
    #133436
    Purpose
    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Bacterial
    Mutation
    C59S/C252A
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133436-plasmid-map-sequence-id-267728

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  49. Flag-TRIM24-F979A/N980A

    Plasmid
    #28142
    Depositor
    Michelle Barton
    Insert
    TRIM24 (TRIM24 Human)
    Tags
    FLAGX2
    Expression
    Mammalian
    Mutation
    F979A and N980A
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  50. IDG_TTYH1_OE_1

    Plasmid
    #161627
    Purpose
    Overexpresses TTYH1
    Depositor
    Mike McManus
    Insert
    TTYH1 (TTYH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    161627-plasmid-map-sequence-id-319553

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Showing: 8751 - 8800 of 167150 results