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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCk1_spacer

Plasmid

#136071

Purpose

Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CaP2AIII_pK18

Plasmid

#153421

Purpose

Source of murine TCR-alpha constant region (TRAC*02) gene followed by P2A

Depositor

Maki Nakayama

Article

Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

murine TCR-alpha constant region (TRAC*02)-P2A

Expression

Bacterial

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ133C2.0

Plasmid

#99893

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131C2.0

Plasmid

#99886

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132C2.0

Plasmid

#99889

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pU6nH1-HD10kb

Plasmid

#172657

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD10kbA/pegRNA-HD10kbB

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLLX13

Plasmid

#79825

Purpose

Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Yeast and bacteria shuttle vector, contains origi…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ132D2.0

Plasmid

#99890

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYIAL31

Plasmid

#60807

Purpose

used to make mutations in yeast POL1

Depositor

Thomas Kunkel, Youri Pavlov

Article

Pavlov et al Genetics. 2001 Sep;159(1):47-64.

Insert

Pol1 (POL1 Budding Yeast)

Expression

Yeast

Mutation

N-terminal part only

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PB-HD118

Plasmid

#172655

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD118A/pegRNA-HD118B

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133D2.0

Plasmid

#99894

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ131D2.0

Plasmid

#100044

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) HIS-tag pET

Plasmid

#8492

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1-unspliced MUT

Plasmid

#242750

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-evoBxb1

Plasmid

#232747

Purpose

IVT template for making modRNA encoding evoBxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

V74A

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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