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pLCHKO_TK1_HPRT1_Lb_4

Plasmid

#155062

Purpose

Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-dsm

Plasmid

#89743

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi dark-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-lsm

Plasmid

#89742

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi lit-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMiR-E6AP-3'UTR-miR-375-mut

Plasmid

#53695

Purpose

Luciferase reporter assay for E6AP 3'UTR with point mutation on miR-375 binding site

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

UBE3A 3'UTR (UBE3A Human)

Use

Luciferase

Mutation

Mutation on miR-375 binding site on E6AP 3'U…

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
p53 S23A targeting vector

Plasmid

#12167

Depositor

Tyler Jacks

Article

MacPherson et al EMBO J. 2004 Sep 15. 23(18):3689-99.

Insert

p53 (Trp53 Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

Ser23 mutation (S23A) in exon 2 and puromycin gen…

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pscAAV-U6-gTgfbr2-CBh-mCherry-SV40late

Plasmid

#249125

Purpose

expression of gTgfbr2 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gTgfbr2 (Tgfbr2 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-KIKO-U6-gOsmr-p2a-mCherry-STOP-pA-400

Plasmid

#249130

Purpose

all-in-one HDRT-based knockin-knockout (KIKO) design to insert mCherry (=knockin) at gOsmr cut site, disrupting Osmr expression

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gOsmr, mCherry (Osmr Synthetic, Mouse)

Use

AAV

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab264-pcDNA3-BRCA1(1314-end)-M1783T-V5-3xFLAG

Plasmid

#249017

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the M1783T mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

M1783T

Promoter

CMV Promoter

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab211-pcDNA3-BRCA1(1314-end)-L1705R-V5-3xFLAG

Plasmid

#249015

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the L1705R mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

L1705R

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab240-pcDNA3-BRCA1(1314-end)-Y1845D-V5-3xFLAG

Plasmid

#249016

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the Y1845D mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845D

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab981-pcDNA3-BRCA1(1314-end)-V1736A-V5-3xFLAG

Plasmid

#249018

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the V1736A mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

V1736A

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab224-pcDNA3-BRCA1(1314-end)-R1699W-V5-3xFLAG

Plasmid

#249019

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the R1699W mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

R1699W

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab223-pcDNA3-BRCA1(1314-end)-R1699Q-V5-3xFLAG

Plasmid

#249020

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the R1699Q mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

R1699Q

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab228-pcDNA3-BRCA1(1314-end)-S1715R-V5-3xFLAG

Plasmid

#249021

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the S1715R mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

S1715R

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab189-pcDNA3-BRCA1(1314-end)-A1708E-V5-3xFLAG

Plasmid

#249022

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the A1708E mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708E

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab521-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-Y1845F-STOP

Plasmid

#249009

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the Y1845F mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845F

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab522-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-Y1845D-STOP

Plasmid

#249010

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the Y1845D mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845D

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab524-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-G1788V-STOP

Plasmid

#249011

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the G1788V mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

G1788V

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab518-pcDNA3-3xFLAG-V5-BRCA1(1314-end)-A1708E-STOP

Plasmid

#249012

Purpose

This plasmid expresses the N-terminally 3x-FLAG tagged BRCT domain that contains the A1708E mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708E

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab979-pcDNA3-BRCA1(1314-end)-A1708V-V5-3xFLAG

Plasmid

#249014

Purpose

This plasmid expresses the C-terminally 3x-FLAG tagged BRCT domain that contains the A1708V mutation

Depositor

Georgios Karras

Article

Gracia et al Cell Rep. 2024 Feb 27;43(2):113803. doi:

Insert

BRCA1(1314aa-end) (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708V

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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