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362 pCS Cherry DEST

Plasmid

#13075

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

Cherry

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe3-RfA-Venus

Plasmid

#186372

Purpose

Destination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST P38KTRDronpa

Plasmid

#205763

Purpose

Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

P38 KTR Dronpa

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-K298M

Plasmid

#140315

Purpose

Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXP CMV/TO DEST (w360-1)

Plasmid

#17386

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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223 pCS EGFP DEST

Plasmid

#13071

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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LifeAct-EGFP-APEX2 in pCSDEST2

Plasmid

#170523

Purpose

Vector for expression of LifeAct APEX2 in mammalian cells. Parton lab clone FTE

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIH CMV/TO DEST (w382-1)

Plasmid

#17394

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-GJA1-20k-N1

Plasmid

#49861

Purpose

Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

GJA1-20k (GJA1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

GJA1-20k corresponds to residues 213 to 382 to Co…

Promoter

CMV

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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