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pME TAEL2.0-5xC120-minpro (JDW 1109)

Plasmid

#224507

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL2.0-5xC120

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-FHC-POLQ-DM

Plasmid

#64880

Purpose

lentiviral expression of human POLQ double mutant (in POL and HDL domains)

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K121M and D2330A,Y2331A

Promoter

EF1alpha

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

Plasmid

#224500

Purpose

Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-mNeonGreen-HA-pA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pFB2D

Plasmid

#195286

Purpose

Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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p5E-dr-myl7-pro (JDW 1148)

Plasmid

#229836

Purpose

A gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

cmlc2/myl7

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

Plasmid

#242589

Purpose

A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh (actin nanobody)

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pThermoCas9_ctrl

Plasmid

#100981

Purpose

Expresses ThermoCas9 and its sgRNA module

Depositor

John van der Oost

Article

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Tags

None

Expression

Bacterial

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

Plasmid

#161580

Purpose

Express Kir2.1 in cells not expressing Cre.

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

Kir2.1-2A-GFP (Kcnj2 Mouse)

Use

AAV

Mutation

NA

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-Rab6a_T27N

Plasmid

#248794

Purpose

Expresses SNAP-Rab6a-T27N (dominant negative) in mammalian cells. Generated from Addgene #46782 plasmid.

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

Rab6a (RAB6A Human)

Tags

SNAPf

Expression

Mammalian

Mutation

Change T 27 to N

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-Rab6a

Plasmid

#248793

Purpose

Expresses SNAP-Rab6a (WT) in mammalian cells. Generated from Addgene #46781 plasmid.

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

Rab6a (RAB6A Human)

Tags

SNAPf

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCIRCNpu

Plasmid

#74226

Purpose

Vector for SICLOPPS-mediated protein circularization using the Nostoc punctiforme DnaE intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

Npu DnaE C-intein and Npu DnaE N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-rhMOG

Plasmid

#225484

Purpose

Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

Depositor

John Pak

Article

Wu et al J Immunol Methods. 2024 Oct 13:113764. d

Insert

Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)

Tags

6xHis tag

Expression

Bacterial

Mutation

deleted amino acids 1-29 (signal peptide) and 150…

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-human TMEM217-PA

Plasmid

#249410

Purpose

Expression vector of human TMEM217 isoform 3 tagged with PA at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Transmembrane protein 217 isoform 3 (TMEM217 Human)

Tags

PA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_HyPer7_cyto

Plasmid

#238967

Purpose

Mammalian neurons HyPer7 expression

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

HyPer7

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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ABCG1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176944

Purpose

Mammalian expression vector encoding ABCG1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ABCG1 (ABCG1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDiCre-mCherry

Plasmid

#164572

Purpose

Integration of a DiCre cassette in PbGFP parasite genome

Depositor

Olivier Silvie

Article

Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

Inserts

FKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pME-DreERT2 (JDW 1344)

Plasmid

#224523

Purpose

A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMYH9-D1424H-FLAG

Plasmid

#183513

Purpose

Gateway expression vector encoding D1424H mutant MYH9 cDNA, with N-terminal Flag tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Aspartic acid 1424 to Histidine

Promoter

CMV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

Plasmid

#92142

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pPB-EF1α-H2B-PA-mCherry-PGKneo

Plasmid

#247340

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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