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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His-ShadowG-iLID

Plasmid

#246942

Purpose

Expresses His-ShadowG-iLID in E.coli

Depositor

Makito Miyazaki

Article

Yamamoto et al Nat Commun. 2025 Aug 26;16(1):7583. doi:

Insert

iLiD

Tags

His-tag and ShadowG

Expression

Bacterial

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCL1023

Plasmid

#252434

Purpose

ASF1A tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

ASF1A (ASF1A Human)

Tags

mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1-unspliced MUT

Plasmid

#242750

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-evoBxb1

Plasmid

#232747

Purpose

IVT template for making modRNA encoding evoBxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

V74A

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1-I87L

Plasmid

#232749

Purpose

IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

I87L

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST17-HO1(51-231)

Plasmid

#203486

Purpose

For recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Arabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FIP200 (1-640)

Plasmid

#203542

Purpose

Make FIP200 NTD:ATG13:ULK1 complex

Depositor

James Hurley

Article

Chen et al Nat Struct Mol Biol. 2025 Sep;32(9):1596

Insert

FIP200 (RB1CC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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Lentiguide-Blast-eNMU710

Plasmid

#172658

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-eNMUA/pegRNA-eNMUB

Use

CRISPR

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCsB_spacer

Plasmid

#136076

Purpose

Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsA_spacer

Plasmid

#136075

Purpose

Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PB-HD252

Plasmid

#172656

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD252A/pegRNA-HD252B

Use

CRISPR

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2 (GC rich)

Plasmid

#115550

Purpose

Multisite gateway vector for 3' tagging with GC-rich APEX2. Used to make 115563. Parton lab clone HHS

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

APEX2 (GC rich)

Use

Multisite gateway entry clone

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT/Acrosin-GFP-GPI

Plasmid

#122141

Purpose

For making acrosin-GFP-GPI RNA

Depositor

Carmen Williams

Article

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Insert

acrosin-GFP-GPI

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Mutation

please view depositor comments below

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode7

Plasmid

#83684

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode7

Use

Retroviral

Mutation

L119P in GFP

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pIVT-p53DD

Plasmid

#232751

Purpose

IVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

p53DD

Use

In vitro transcription

Mutation

deletion of DNA binding domain

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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psPAX2-D64V

Plasmid

#63586

Purpose

psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

Depositor

David Rawlings, Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Packaging plasmid for integrase deficient lentiviral vectors

Use

Integrase deficient lentiviral packaging vector

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCBC-DT1T2

Plasmid

#50590

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T1, U626t plus, U629p, T2

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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