We narrowed to 79,059 results for: Mycs;

1. pAV0749

   Plasmid

   #133483

   Purpose

   Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJM126

   Plasmid

   #49339

   Purpose

   bacterial expression of RFA1, RFA2, and RFA3

   Depositor

   Steven Brill

   Article

   He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

   Inserts

   RFA3 (RFA3 Budding Yeast)
   RFA2 (RFA2 Budding Yeast)
   RFA1 (RFA1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAV0758

   Plasmid

   #133511

   Purpose

   Expression of 3GFP tagged RasAct probe to monitor active Ras1

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV0783

   Plasmid

   #133515

   Purpose

   Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-2T FRB S2035I

   Plasmid

   #26608

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   FRB domain only (aa 2015-2114); S2035I

   Promoter

   Available since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pAV0714

   Plasmid

   #133479

   Purpose

   Expression in fission yeast from promoter pact1, integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCfB9337 (pgRNA_IV-1_NatMX)

   Plasmid

   #161590

   Purpose

   EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site IV-1

   Depositor

   Irina Borodina

   Article

   Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

   Insert

   guiding RNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZA31

   Plasmid

   #158509

   Purpose

   In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   ZAR1-4xMyc
   

   Use

   Tags

   4xMyc

   Expression

   Plant

   Mutation

   L17E

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV0612

   Plasmid

   #133505

   Purpose

   Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMB-TGN61r

    Plasmid

    #61175

    Purpose

    Fluorescent reporter for trans-Golgi network mCherry-MtSYP61 expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtSYP61
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKH-MoHyPer

    Plasmid

    #78267

    Purpose

    Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

    Depositor

    Nicole Donofrio, Jeffrey Caplan

    Article

    Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

    Insert

    MoHyper
    

    Use

    Express in magnaporthe

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Rp27

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pAV0750

    Plasmid

    #133484

    Purpose

    Expression in fission yeast from promoter purg1 , integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(urg1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-TRP1-PGAL1-GFP

    Plasmid

    #41615

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP(S65T), TRP1 reverse orientation, and yeast AD…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV0756

    Plasmid

    #133508

    Purpose

    Expression of 3mCherry tagged CRIB domain to monitor active Cdc42

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(act1)-CRIB(gic2aa2-181)-3mCherry-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV0764

    Plasmid

    #133520

    Purpose

    Expression of mCherry targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-mCherry-AHDL-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV0773

    Plasmid

    #133519

    Purpose

    Expression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMB-TMEg

    Plasmid

    #61209

    Purpose

    Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-GFP-BCP
    

    Use

    Tags

    EGFP

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV0835

    Plasmid

    #133512

    Purpose

    Expression of mCherry tagged CHD domain to monitor actin cytoskeleton

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-mCherry-CHDRng2-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YIp211-P30-His-pol30(K127/164R)-3'

    Plasmid

    #99549

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTJK517

    Plasmid

    #121474

    Purpose

    CEN TRP1 CNA1

    Depositor

    Tami Kingsbury

    Article

    Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

    Insert

    CNA1
    

    Use

    Yeast cen vector

    Tags

    Expression

    Mutation

    Promoter

    Endogenous genomic promoter region

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Dom34+GST

    Plasmid

    #19099

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag, GST, and N tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pAV0816

    Plasmid

    #133510

    Purpose

    Expression of mTagBFP2 tagged CRIB domain to monitor active Cdc42

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(act1)-CRIB(gic2aa2-181)-mTagBFP2-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-TRP1-PGAL1-GST

    Plasmid

    #41612

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GST, TRP1 reverse orientation, and yeast ADH1 ter…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-2T FRB R2109A

    Plasmid

    #26609

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    FRB domain only (aa 2015-2114); R2109A

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

26. YIplac204-TDH3p-CDC19-CYC1t

    Plasmid

    #216122

    Purpose

    Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

    Depositor

    Yvonne Nygård

    Article

    Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

    Insert

    Pyruvate kinase 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3 promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CPY-ymsfGFP

    Plasmid

    #209538

    Purpose

    Expresses CYP tagged with ymsfGFP in S. cerevisiae

    Depositor

    Patrick Lajoie

    Article

    Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

    Insert

    CPY
    

    Use

    Tags

    ymsfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CPY*-ymsfGFP

    Plasmid

    #209539

    Purpose

    Expresses the misfolded CPY* mutant tagged with ymsfGFP in S. cerevisiae

    Depositor

    Patrick Lajoie

    Article

    Chadwick et al Mol Biol Cell. 2024 Dec 11:mbcE24040147.

    Insert

    CPY*
    

    Use

    Tags

    ymsfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEM_Δflv3.sm

    Plasmid

    #185533

    Purpose

    a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus

    Depositor

    Paula Tamagnini

    Article

    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

    Insert

    aadA gene flanked by flv3 upstream and downstream regions
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3-2xMARS-PLD

    Plasmid

    #205237

    Purpose

    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

    Depositor

    Jeremy Baskin

    Inserts

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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