We narrowed to 4,990 results for: mcs

1. pJT331 Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast

   Plasmid

   #195538

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-Q+N+Z+F-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

   Plasmid

   #195540

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT342 Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

   Plasmid

   #195541

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dCas9-3XFLAG-RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   pEF1a

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIG-748_HA-GD2-28z_CAR_BATF

   Plasmid

   #207488

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-805_HA-GD2-28z_CAR_BATF-RFP

   Plasmid

   #207492

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF-RFP, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-755_HA-GD2-28z_CAR_RFP-JUN

   Plasmid

   #207494

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP-JUN, HA-GD2-28z_CAR (JUN Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-837_NY-ESO-1_TCR_tFAS

   Plasmid

   #207500

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tFAS, NY-ESO-1_TCR (FAS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209061

   Purpose

   Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

   Insert

   KDM6A
   

   Use

   Gateway vector to be used for lr reaction

   Promoter

   U6

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209062

   Purpose

   Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

   Insert

   non-targeting sgRNA (C0111)
   

   Use

   Gateway vector to be used for lr reaction

   Promoter

   U6

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1-FRB-MCU-Flag-TetOFF

    Plasmid

    #185659

    Purpose

    Tet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitope

    Depositor

    Dipayan Chaudhuri

    Article

    Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

    Insert

    Mitochondrial calcium uniporter (MCU Human)
    

    Use

    Lentiviral

    Tags

    FRB and Flag

    Expression

    Mammalian

    Promoter

    pTight TRE

    Available Since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. t1-405(del30)

    Plasmid

    #166128

    Purpose

    Expression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-405(del30) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-405, with 30 amino acid deletion in th…

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. t1-405(T144E,T150E)

    Plasmid

    #166130

    Purpose

    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    HuT1head1-405(T144E,T150E) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-405, mutations T144E and T150E

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

    Plasmid

    #156413

    Purpose

    kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    EYFP

    Promoter

    kdrl

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

    Plasmid

    #156404

    Purpose

    Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mTagBFP2

    Mutation

    mutant KRAS4A-G12D

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINTO-N3::hTET3 V908L

    Plasmid

    #136370

    Purpose

    Expresses epitope-tagged human TET3 V908L variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    V908L

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINTO-N3::hTET3 V1089M

    Plasmid

    #136369

    Purpose

    Expresses epitope-tagged human TET3 V1089M variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2x Strep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    V1089M

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINTO-N3::hTET3 A1076T

    Plasmid

    #136368

    Purpose

    Expresses epitope-tagged human TET3 A1076C variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    A1076T

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pINTO-N3::hTET3 R752C

    Plasmid

    #136367

    Purpose

    Expresses epitope-tagged human TET3 R752C variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    R752C

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pINTO-N3::hTET3 F1072C

    Plasmid

    #136366

    Purpose

    Expresses epitope-tagged human TET3 F1072C variant in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    F1072C

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-FLAG-RPS14(89-151)

    Plasmid

    #122299

    Purpose

    expresses a part of RPS14 protein with a flag tag

    Depositor

    Gerardo Ferbeyre

    Article

    Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

    Insert

    RPS14 (RPS14 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1 to 88

    Promoter

    CMV

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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