We narrowed to 7,509 results for: PAC;

1. pU6-Universal

   Plasmid

   #52694

   Purpose

   This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

   Depositor

   Sebastian Lourido

   Inserts

   Cas9
   Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
   

   Use

   CRISPR; Toxoplasma gondii

   Tags

   3X FLAG and Nuclear localization signal

   Expression

   Mutation

   Promoter

   TgTUB1 and Toxoplasma U6

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SauriBE4max

   Plasmid

   #135969

   Purpose

   Expresses AncBE4max and nSauriCas9

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-TRBP

   Plasmid

   #15666

   Depositor

   Dong-Yan Jin

   Article

   Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

4. GZnP2 in pcDNA3.1+

   Plasmid

   #161739

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

   Insert

   GZnP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD143

   Plasmid

   #119880

   Purpose

   Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pBAD_dCas9sth1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PH-PLCd1-mNGx1

   Plasmid

   #202751

   Purpose

   expresses fluorescent protein-tagged lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   PLCD1v2(1-170):mNG2 (PLCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TagBFP2- PIP4K2BD278K

   Plasmid

   #202729

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-Rap1b V12

   Plasmid

   #118323

   Purpose

   Expression of bovine Rap1b

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   Rap1b V12 (RAP1B Bovine)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-Rap1b N17

   Plasmid

   #118322

   Purpose

   Expression of bovine Rap1b

   Depositor

   Philip Stork

   Article

   Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

   Insert

   Rap1b N17 (RAP1B Bovine)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S17N

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ttAtCas12a+int

    Plasmid

    #182384

    Purpose

    Gives very high editing efficiency in barley. Also works in Brassica oleracea

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al bioRxiv 2022.04.28.489853

    Insert

    LbCas12a coding sequence with D156R and Arabidopsis introns
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R + 8 Arabidopsis introns added

    Promoter

    None

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pReporter_12

    Plasmid

    #60572

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. SauriABEmax

    Plasmid

    #135968

    Purpose

    Expresses ABEmax and nSauriCas9

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB8_4-3i-ATP8_v8_AGKS

    Plasmid

    #191513

    Purpose

    Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8 ZF-DdCBE 4-3i-ATP8 AGKS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB5_R8-3i-ATP8_v8_AGKS

    Plasmid

    #191510

    Purpose

    Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8 ZF-DdCBE R8-3i-ATP8 AGKS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG198 PRKD1 3'UTR

    Plasmid

    #12010

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    PRKD1 3'UTR (PRKD1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116954

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM197

    Plasmid

    #132665

    Purpose

    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

    Depositor

    Joseph Sorg

    Article

    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

    Insert

    Upstream & Downstream pyrE deletion region
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LbCas12a-gRNA_BbsI_CAM

    Plasmid

    #183222

    Purpose

    LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-APLO

    Plasmid

    #112805

    Purpose

    gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

    Depositor

    Chun Han

    Article

    Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. iRFP670-PIP5K1A

    Plasmid

    #202749

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP670:PIP5K1A (PIP5K1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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