We narrowed to 4,225 results for: pCMV

1. MMLV-RT(dRH) for Split-PE (pEH50)

   Plasmid

   #190106

   Purpose

   Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT(delta RNAseH)
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   mutations from RT in PE2 and truncation of RNAse …

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag- MCL1-3A (S159, S162, T163 mutated to A)

   Plasmid

   #197458

   Purpose

   MCL-1 phosphodegron mutants

   Depositor

   Omar Khan

   Article

   Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

   Insert

   MCL1 (MCL1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S159A, S161A, T163A

   Promoter

   CMV

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCas9 PE2*

   Plasmid

   #169850

   Purpose

   Mammalian Expression, SpyCas9 prime editor, optimized NLS

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   spCas9 PE*
   

   Tags

   bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. rnLbCas12a-PE2

   Plasmid

   #213746

   Purpose

   For circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   rnLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, H759A, R1138A in LbCas12a

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHA-mLRH-1

   Plasmid

   #16342

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 May . 25(9):3492-505

   Insert

   LRH-1 (Nr5a2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

6. ND4 right TALE–G1397 DddAtox C

   Plasmid

   #158097

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ND4 left TALE–G1397 DddAtox N

   Plasmid

   #158094

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. G1333 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157837

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9 TadCBEe

   Plasmid

   #193836

   Purpose

   Express TadCBEe (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDe-SpCas9D10A-2xUGI
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. xCas9(3.6)-ABE(7.10)

    Plasmid

    #108387

    Purpose

    Mammalian expression vector for xCas9(3.6)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-ABE(7.10)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frt-hspB7

    Plasmid

    #63098

    Purpose

    Expression of HSPB7 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb7 (HSPB7 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pR2Q024y

    Plasmid

    #101022

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    cmV

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

    Plasmid

    #207634

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
    

    Expression

    Mammalian

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpCas9 TadCBEa-V106W

    Plasmid

    #193838

    Purpose

    Express TadCBEa-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDa V106W-SpCas9 D10A
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SauCas9 PE2*

    Plasmid

    #169851

    Purpose

    Mammalian Expression, sauCas9 based prime editor

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SaCas9-N580A-M-MLV
    

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ND4 right TALE–G1333 DddAtox C

    Plasmid

    #158095

    Purpose

    expresses the right-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ND4 left TALE–G1333 DddAtox N

    Plasmid

    #158093

    Purpose

    expresses the left-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-N–1xUGI
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)

    Plasmid

    #221998

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. dxCas(3.6)-VPR

    Plasmid

    #108388

    Purpose

    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.6)-VPR
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1-surrogate-reporter

    Plasmid

    #65624

    Purpose

    Fluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attP
    

    Use

    Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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