164,884 results

1. pET_RTX_(exo-)

   Plasmid

   #102786

   Purpose

   Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

   Depositor

   Andrew Ellington

   Article

   Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

   Insert

   reverse transcription xenopolymerase with 3'-5' deficient exonuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRZero

   Plasmid

   #120275

   Purpose

   Blunt-end cloning vector of PCR-products using ZERO-backgound system

   Depositor

   Ken Motohashi

   Article

   Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Grp1-PH pEGFP-C1

   Plasmid

   #71378

   Purpose

   For analysis of PH domain localization in mammalian cells

   Depositor

   Mark Lemmon

   Article

   Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Pleckstrin homology (PH) domain; aa 247-399

   Promoter

   CMV

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC1_oROS-HT

   Plasmid

   #216413

   Purpose

   Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZac2.1 gfaABC1D-cyto-GCaMP8f

   Plasmid

   #186050

   Purpose

   AAV-mediated expression of GCaMP8f under the control of GfaABC1D promoter

   Depositor

   Loren Looger

   Insert

   jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKDsgRNA-ack

   Plasmid

   #62654

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. ORFtag_TetR-SD_Frame1

   Plasmid

   #220981

   Purpose

   ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

   Depositor

   Alexander Stark

   Insert

   TetR repressor
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK promoter

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. miniCGBE1-SpRY (pBM1571)

   Plasmid

   #170105

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

   Promoter

   CMV

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRT

    Plasmid

    #120274

    Purpose

    TA-cloning vector of PCR-products

    Depositor

    Ken Motohashi

    Article

    Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge