165,971 results

1. pEB1deltaC-mCherry

   Plasmid

   #80603

   Purpose

   Expresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus end

   Depositor

   Torsten Wittmann

   Article

   Ettinger et al Curr Biol. 2016 Jun 20;26(12):1549-55. d

   Insert

   EB1 (MAPRE1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Truncated C-terminus; EB1 coding sequence ends at…

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVTNT-T7-KPNA2

   Plasmid

   #26678

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 2 (KPNA2 Human)
   

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPAGFP-N1

   Plasmid

   #11909

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   pPAGFP-N1
   

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBlueSKMimRNA

   Plasmid

   #102535

   Purpose

   Template for synthesis of Minos transposase mRNA

   Depositor

   Michalis Averof

   Article

   Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

   Insert

   Minos transposase mRNA
   

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hsyn-GRAB_DA2m

   Plasmid

   #140553

   Purpose

   Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EGFP-C1-centrin-1

   Plasmid

   #73331

   Purpose

   Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag

   Depositor

   Manuel Thery

   Insert

   centrin-1 (CETN1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-GCN5

   Plasmid

   #65386

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   GCN5 (KAT2A Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. BACE2_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176942

   Purpose

   Mammalian expression vector encoding BACE2 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   BACE2 (BACE2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB CAG-sfGFP

   Plasmid

   #133568

   Purpose

   CAG sfGFP piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   sfGFP
   

   Expression

   Mammalian

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-FGFR3

    Plasmid

    #116742

    Purpose

    Lentiviral expression of FGFR3

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ER-RA

    Plasmid

    #209872

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GluA1-scFV-Halo

    Plasmid

    #230065

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOpen-SfiIR (cDNA)

    Plasmid

    #165528

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    SfiI (used to create cDNA libraries)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. SpyTag003-mKate2

    Plasmid

    #133452

    Purpose

    Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mKate2
    

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Sport6-CD9-pHluorin

    Plasmid

    #130905

    Purpose

    Expression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.

    Depositor

    DM Pegtel

    Article

    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

    Insert

    CD9-pHluorin (CD9 Aequorea victoria, Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    phluorin inserted between Asn-50 and Asn-51 in ex…

    Promoter

    CMV

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX302-MKP5-V5 puro

    Plasmid

    #87770

    Purpose

    Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-lsMSP1D1

    Plasmid

    #240151

    Purpose

    Expression of the MSP 'His-lsMSP1D1'

    Depositor

    Lise Arleth

    Article

    Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

    Insert

    His-lsMSP1D1
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB_EF1a_tNGFR_ZeoR

    Plasmid

    #212648

    Purpose

    piggyBac vector to express tNGFR with zeocin resistance

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    tNGFR
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-rxRFP

    Plasmid

    #67830

    Purpose

    Redox-Sensitive Red Fluorescent Protein

    Depositor

    Huiwang Ai

    Article

    Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

    Insert

    Redox-Sensitive Red Fluorescent Protein
    

    Tags

    His Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. pS648∙CsR

    Plasmid

    #197860

    Purpose

    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    gRNA scaffold - Gm resistance cassette - incP oriT
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PEM7

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDonor_Tn[himar1C9]

    Plasmid

    #213906

    Purpose

    Mariner transposon with ATc-inducible himar1C9 transposase

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with cam resistance and ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBS-Int

    Plasmid

    #50000

    Purpose

    Expresses the integrase on a suicide plasmid

    Depositor

    Brian Robertson, Peter Sander

    Article

    Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

    Insert

    Integrase from pMV361
    

    Expression

    Bacterial

    Promoter

    own

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pEGFP-N3/hArf6(WT)-EGFP

    Plasmid

    #79423

    Purpose

    Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pENTR223.1_mmDdx58 (closed)

    Plasmid

    #160107

    Purpose

    Expresses murine Ddx58 in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Ddx58 (Rigi Mouse)
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLVX-CMV-Galpha-oA ONE-GO

    Plasmid

    #189732

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-oA ONE-GO
    GNAO (Gnao1 Rat)
    

    Use

    Lentiviral

    Mutation

    I250 (silent mutation in YFP)

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. pKK-MBP-TEV

    Plasmid

    #105771

    Purpose

    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    MBP-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHD-DsRed

    Plasmid

    #51434

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

    Depositor

    Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    LoxP-3xP3-DsRed-LoxP
    

    Promoter

    3xP3

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pNF02-mLychee

    Plasmid

    #219400

    Purpose

    Constitutive expression of mLychee in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mLychee
    

    Expression

    Bacterial

    Promoter

    proDp

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTM

    Plasmid

    #162783

    Purpose

    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Type

    Empty backbone

    Tags

    10xHis, c-myc, and hTPA leader

    Expression

    Mammalian

    Promoter

    CHO Elongation factor 1A

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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