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1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA

Plasmid

#109317

Purpose

Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA

Depositor

William Lagor

Publication

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG042

Plasmid

#113998

Purpose

Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Tetracycline

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-T7-Top-T

Plasmid

#87309

Purpose

Expresses the "Top" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Top" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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LI D-E GFP + linker c3

Plasmid

#54410

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + GFP

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCP5b

Plasmid

#37185

Depositor

Adam Bogdanove

Publication

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Type

Empty backbone

Expression

Yeast

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HIS-Ub-K48R

Plasmid

#221368

Purpose

Expresses 6xHIS-tagged Ubiquitin containing K48R mutation in mammalian cells

Depositor

Aga Gambus

Publication

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

Ubiquitin

Tags

6xHIS

Expression

Mammalian

Mutation

Changed Lysine 48 to Arginine

Promoter

CMV

Available since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D HRP-TM

Plasmid

#231499

Purpose

Expressing HRP on the cell surface of astrocytes

Depositor

Baljit Khakh

Publication

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

HRP

Use

AAV

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT11.B

Plasmid

#201005

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

TagBFP

Expression

Bacterial

Promoter

J23104

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRβεζ

Plasmid

#187353

Purpose

Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Depositor

Simon Davis

Publication

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Use

Lentiviral

Promoter

SFFV

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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YIplac211-iGFP-TLG2

Plasmid

#105262

Purpose

Plasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg2 (TLG2 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Reb_140 aka PAS625

Plasmid

#71230

Purpose

Reb_1 with RebA(S88P)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(S88P)

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pDGB1_omega1

Plasmid

#68234

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pICH89921

Plasmid

#48072

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pBS Rad

Plasmid

#11236

Depositor

Ronald Kahn

Publication

Reynet et al Science. 1993 Nov 26. 262(5138):1441-4.

Insert

Rad (RRAD Human)

Expression

Bacterial

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only

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pTW334i

Plasmid

#237319

Purpose

expresses A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1

Depositor

Tina Wang

Publication

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

A. thaliana Cpn60α Cpn60β Cpn20 Raf2 RbcX Bsd2 N. tabacum Raf1

Expression

Bacterial

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pKH144_mCherry-YIPF4 WT

Plasmid

#214972

Purpose

mCherry tagged YIPF4

Depositor

Wade Harper

Publication

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mCherry-YIPF4 (YIPF4 Human)

Use

Lentiviral

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135978

Purpose

gRNA against the right loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA2

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Promoter

CB

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pOGG014

Plasmid

#113988

Purpose

Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Endlinker/terminator Part 4

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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