We narrowed to 4,131 results for: pCMV

1. pCDNA_B2AR-i-pep

   Plasmid

   #47436

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

2. nCre-ST-NLS-P2A-NLS-pSC-cCre

   Plasmid

   #207638

   Purpose

   A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SpyCatcher’s critical lysine

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pS6-hα2M-H6

   Plasmid

   #128495

   Purpose

   Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (G27-S1468) (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-IFITM3-F8-TAG

   Plasmid

   #122475

   Purpose

   expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Phenylalanine 8 to amber codon

   Promoter

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPACE-ΔUGI (pRZ1836)

   Plasmid

   #140243

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Frt-V5-hspB4

   Plasmid

   #63105

   Purpose

   Expression of HSPB4 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb4 (CRYAA Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Frt-V5-hspB6

   Plasmid

   #63107

   Purpose

   Expression of HSPB6 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb6 (HSPB6 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyCas9 PE2*-3HA

   Plasmid

   #169854

   Purpose

   Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-star-M-MLV-3HA
   

   Use

   Tags

   3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AID'-BE4max

   Plasmid

   #174696

   Purpose

   Base Editing

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

   Insert

   AID'-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   UGI-E11K

   Promoter

   CMV

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Frt-hspB1

    Plasmid

    #63092

    Purpose

    Expression of HSP1 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb1 (HSPB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA_B2AR-no-pep

    Plasmid

    #47439

    Purpose

    control FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

12. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SECURE BE4max(R33A) (pBM608)

    Plasmid

    #140251

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-SUMO-A'-l4-G'-IRES-EmGFP

    Plasmid

    #209127

    Purpose

    Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'-l4-G'-IRES-EmGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-SUMO-A'-l4-G-IRES-EmGFP

    Plasmid

    #209126

    Purpose

    Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'-l4-G-IRES-EmGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid

    #123616

    Purpose

    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

    Insert

    NLS-nCas9-NLS-P2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A in SpCas9

    Promoter

    CMV

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2xmyc-RAB35

    Plasmid

    #164632

    Purpose

    Expresses RAB35 in mammalian cells with a 2xmyc tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    RAB35 (RAB35 Human)
    

    Use

    Tags

    2xmyc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPKm-105

    Plasmid

    #104853

    Purpose

    pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PhyB NT GBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA_B2AR-q-pep

    Plasmid

    #47437

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Frt-hspB8

    Plasmid

    #63099

    Purpose

    Expression of HSPB8 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb8 (HSPB8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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