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pETM6_Ptac_BmHps_BmPhi_BsMdh

Plasmid

#129103

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

Depositor

Eleftherios Terry Papoutsakis

Publication

Whitaker et al Metab Eng. 2017 Jan;39:49-59. doi: 10.10

Insert

BsMDH BmHPS BmPHI

Expression

Bacterial

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57mini ZM249M P90A _env eGFP

Plasmid

#132939

Purpose

pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

Depositor

Jeremy Luban

Publication

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

HIV-1ZM249M provirus (P90A) with env deletion, GFP

Use

Lentiviral

Mutation

P90A

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-GFPFANACfullWT

Plasmid

#126551

Purpose

AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

Depositor

Rory McQuiston

Publication

Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

Insert

eGFP N-terminal fused to FMRFamide gate sodium channel

Use

AAV

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-FullLength-TET1-CXXC/LLAA

Plasmid

#124399

Purpose

Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Publication

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)

Tags

Flag

Expression

Mammalian

Mutation

CXXC zinc finger deletion (aa 585-624) and L897A/…

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pJZ001

Plasmid

#120230

Purpose

Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

Depositor

Bradley Moore

Publication

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

intB13 integrase casette

Expression

Bacterial

Mutation

bla-g717a (amp resistance gene)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCF204

Plasmid

#121656

Purpose

U6-sgRNA EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Publication

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

Cas9

Use

Lentiviral

Promoter

U6

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF226

Plasmid

#121667

Purpose

EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Publication

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

cas9

Use

Lentiviral

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-GCN4-dCEN-INCENP

Plasmid

#108510

Purpose

expression of vsv-GCN4-dCEN-INCENP

Depositor

Susanne Lens

Publication

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCN4-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

replace CEN box for GCN4 dimerazation domain

Promoter

CMV

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR3-vsv-CenpB 1-166-Sgo1 N61I

Plasmid

#108498

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Publication

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106353

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

Depositor

Stephen Tapscott

Publication

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

D4Z4 gRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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FGFR3_HUMAN_D0

Plasmid

#79731

Purpose

This plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FGFR3 (FGFR3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
EPHB3_HUMAN_D0

Plasmid

#79732

Purpose

This plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB3 (EPHB3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
SLK_HUMAN_D0

Plasmid

#79733

Purpose

This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

SLK (SLK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
PHKG2_HUMAN_D0

Plasmid

#79736

Purpose

This plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PHKG2 (PHKG2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
VRK2_HUMAN_D0

Plasmid

#79737

Purpose

This plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK2 (VRK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KAPCA_HUMAN_D0

Plasmid

#79741

Purpose

This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KAPCA

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
BMPR2_HUMAN_D0

Plasmid

#79709

Purpose

This plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

BMPR2 (BMPR2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2G_HUMAN_D0

Plasmid

#79711

Purpose

This plasmid encodes the kinase domain of KCC2G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2G (CAMK2G Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK01_HUMAN_D0

Plasmid

#79713

Purpose

This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK01 (MAPK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2B_HUMAN_D0

Plasmid

#79718

Purpose

This plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Publication

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2B

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only