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Plasmid#127872PurposeDual expression vector for mitochondrial matrix and outer membrane Ca2+ sensorsDepositorInsertOuter membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human PNP his-tagged
Plasmid#64076PurposeExpression of human Purine Nucleoside PhosphorylaseDepositorInsertPurine Nucleoside Phosphorylase (PNP Human)
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
human PNP non-tagged
Plasmid#64199PurposeExpression of native human PNP (non-tagged)DepositorInsertPurine Nucleoside Phosphorylase (PNP Human)
UseTagsnoneExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pScalps Luciferase Zsgreen
Plasmid#184784PurposeLentiviral vector with luciferase gene under SFFV promoter and Zsgreen under Cyclophilin promoterDepositorInsertFirefly Luciferase and Zsgreen
UseLentiviral and LuciferaseTagsExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
human MTAP
Plasmid#64077PurposeExpression of human 5'-methylthioadenosine phosphorylaseDepositorInsert5'-methylthioadenosine phosphorylase (MTAP Human, Synthetic)
UseTagsHistidine tag and TEVExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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His-DERA
Plasmid#203225PurposeFor protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxyribose aldolase
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF.DEST51-mVenus
Plasmid#154899PurposeExpresses a fluorescent protein, mVenusDepositorInsertmVenus
UseTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmGFP
Plasmid#188451PurposeExpression of a modified GFP (mGFP) with short half-lifeDepositorInsertCyclin destruction box
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C2
Plasmid#141247PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HGXPRT
Plasmid#64201PurposeExpression of Plasmodium falciparum HGXPRTDepositorInsertHypoxanthine guanine xanthine phosphoribosyltransferase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPT-TAR C1
Plasmid#141246PurposeTransformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C1
Plasmid#141248PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertReduced Mitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
S. aureus DHPS
Plasmid#64079PurposeExpression of Staphylococcus aureus dihydropteroate synthaseDepositorInsertdihydropteroate synthase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
His-AgdNK
Plasmid#203226PurposeFor protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSirt3-RFP
Plasmid#216806PurposeHuman Sirt3-RFP driven by the CaMKII promoterDepositorInsertSirt3 (SIRT3 Human)
UseLentiviralTagsmRFP1ExpressionMammalianMutationPromoterCamKIIAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rad51–Cas9(D10A)
Plasmid#125567PurposeExpresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTZ tRNA_gRNA empty scaffold
Plasmid#188450PurposeExpression of gRNAs using a human tRNA promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotertRNA glutamineAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
rSirt3 shRNA (pLKO.1 vector)
Plasmid#216807PurposeshRNA against rat Sirt3DepositorInsertSirt3 (Sirt3 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only