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pKA-141

Plasmid

#79832

Purpose

Expresses RpBphP1-mCherry

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

BphP1-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pT2/SVNeo-103

Plasmid

#79841

Purpose

Expresses RpBphP1-mCherry-TetR under CMVd1 promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

RpBphP1-mCherry-Intersectin1 DHPH domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pKA-144

Plasmid

#79839

Purpose

Expresses RpBphP1-mCherry-Intersectin1 DHPH domain under CMVd1

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

RpBphP1-mCherry-Intersectin1 DHPH domain

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRT_TO_TurboID-mCherry-Cry2

Plasmid

#166504

Purpose

Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

Depositor

Angelos Constantinou

Article

Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

Insert

None

Use

Tags

TurboID and mCherry-CRY2

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1 (M50P)

Plasmid

#178651

Purpose

Mammalian expression of myc-tagged MR1 (M50P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

M50P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (H83P)

Plasmid

#178652

Purpose

Mammalian expression of myc-tagged MR1 (H83P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

H83P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (Q153P)

Plasmid

#178653

Purpose

Mammalian expression of myc-tagged MR1 (Q153P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Q153P

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-CERT-PH

Plasmid

#49572

Purpose

expresses the plekstrin homology domain of CERT/OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

GPBP (CERT1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

expresses AA 1-122

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-CERT PH domain

Plasmid

#58725

Purpose

Expresses GFP-Tagged CERT PH domain in yeast

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-CD16.7-hPC1-eGFP-3HA

Plasmid

#108401

Purpose

Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Use

Tags

eGFP-3HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

Plasmid

#108404

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Human)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-eGFP

Plasmid

#108394

Purpose

Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

variant ID rs13468401 missense variant R4237Q

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_V4146I-eGFP

Plasmid

#108405

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely non-pathogenic variant.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPC1_MTS_L4137P-eGFP

Plasmid

#108403

Purpose

Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

PKD1 (PKD1 Mouse)

Use

Tags

3xHA and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-FLAG-CIP2A FL (sg2R)

Plasmid

#222624

Purpose

Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1-alpha promoter

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-Flag-CIP2A

Plasmid

#222623

Purpose

mammalian expression vector of Flag-tagged CIP2A

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)

Plasmid

#222625

Purpose

Lentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Deletion of the residues 561-625 (ΔNES)

Promoter

EF-1-alpha promoter

Available since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGL1_105

Plasmid

#210689

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Insert

pBP1 ORI with barcode 11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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