2,226 results

1. pRluc-LC3G120A

   Plasmid

   #105003

   Purpose

   Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Glycine 120 mutated to alanine, prevents lipidati…

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

   Plasmid

   #196224

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ
   

   Use

   AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

   Tags

   IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS426GFP-2×PH(PLCδ)

   Plasmid

   #36092

   Depositor

   Scott Emr

   Article

   Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

   Insert

   2xPH(PLCdelta) fusion
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   contains aa11-140 of PLCδ (including the PH domai…

   Promoter

   PRC1

   Available since

   June 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

   Plasmid

   #71878

   Purpose

   For mitochondrial unfolded protein response research

   Depositor

   Nicholas Hoogenraad

   Article

   Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

   Insert

   OTC-delta (Otc Rat)
   

   Use

   Tags

   Expression

   Mutation

   Deleted amino acid 30-114 from full length OTC

   Promoter

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRluc-LC3wt

   Plasmid

   #105002

   Purpose

   Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-SypHluorin 4x (S4x)

   Plasmid

   #37005

   Depositor

   Yongling Zhu, Stephen Heinemann

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin 4x (Syp Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Four copies of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1-TrkB

   Plasmid

   #32500

   Depositor

   Rosalind Segal

   Insert

   TRKB (Ntrk2 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. G protein alpha-s-CFP

   Plasmid

   #55793

   Purpose

   G protein alpha-s internally tagged with ECFP and EE epitope

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

   Insert

   G-alpha-s-EE-ECFP (Gnas Rat)
   

   Use

   Tags

   ECFP flanked by SGGGS on each side was inserted i…

   Expression

   Mammalian

   Mutation

   His was substituted for Asn164 in ECFP (Clontech)…

   Promoter

   CMV

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherryC2-Gephyrin P1

   Plasmid

   #68820

   Purpose

   mCherry tagged Geph for mammalian expression

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al Proc Natl Acad Sci U S A. 2011 Jan 4;108

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyrin…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG.PKCzeta

    Plasmid

    #10799

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-TRE-GCaMP6s

    Plasmid

    #135418

    Purpose

    Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.

    Depositor

    Rylan Larsen

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Vamp3-pHluorin

    Plasmid

    #190152

    Purpose

    Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp3 Rat)
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-6p1-GST-ArcFL

    Plasmid

    #119877

    Purpose

    Bacterial Expression construct for protein production and purification

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

    Plasmid

    #89508

    Purpose

    Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

    Insert

    rAPOBEC1-nCas9 (D10A)-UGI-NLS
    

    Use

    Tags

    UGI and rAPOBEC-XTEN

    Expression

    Bacterial

    Mutation

    D10A mutation of Cas9

    Promoter

    T7

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE Gria1-GFP KI

    Plasmid

    #131489

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. bactin:GFP-LAP2b

    Plasmid

    #105980

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    LAP2b
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-CIB1-mCerulean-MP

    Plasmid

    #58366

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Kif5A-GFP-CIBN

    Plasmid

    #102252

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG.PKCzeta.K/W

    Plasmid

    #10800

    Depositor

    Alex Toker

    Article

    Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

    Insert

    PKC zeta (Prkcz Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Kinase dead mutant: K281W (Lys 281 to Trp)

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pZac2.1-GfaABC1D-lck-jGCaMP8s

    Plasmid

    #176761

    Purpose

    AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Has Service

    AAV1 and AAV9

    Insert

    lck-jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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