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Plasmid#210583PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase- pADepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_SM-FLuc100-pA
Plasmid#210584PurposeExpresses SM(FLAG)-optimal Firefly luciferase - pADepositorInsertSpaghetti monster (FLAG)-optimal FLuc
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dm_λHA-FLuc0_CSC
Plasmid#210589PurposeExpresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)DepositorInsertLambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)
UseLuciferaseTagslambda HAExpressionInsectMutationPromoterAc5Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-EBFP2-FRB
Plasmid#222417PurposeTo express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertCacng2 (Cacng2 Mouse)
UseTagsEBFP2-HA-FRBExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-LAP2beta
Plasmid#222416PurposeTo express LAP2beta with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLAP2beta (Tmpo Rat)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-BAF
Plasmid#222414PurposeTo express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertBAF (BANF1 Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-LYS-IV
Plasmid#218118PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80-421)
Plasmid#209005PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(96-421)
Plasmid#209006PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 96-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(143-421)
Plasmid#209007PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET (SUMO)-Syt9
Plasmid#195713PurposeBacterial expression plasmid encoding SUMO-tagged full-length Syt9DepositorInsertSyt9 (Syt5 Rat)
UseTagsGST-SUMOExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
ARP3-V5-TurboID
Plasmid#195144Purposemeasure COR1C interaction boichemicallyDepositorInsertACTR3 (ACTR3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
COR1C CC-Halo
Plasmid#195146Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
UseTagsExpressionMammalianMutationtruncated proteinPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13D1-1576-(Y768A)
Plasmid#194006PurposeY to A mutation in VPS13D geneDepositorInsertCodon optimized VPS13D (VPS13D Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
VPS13D1-1576-(Y1253S)
Plasmid#194005PurposeY to S mutation in VPS13D geneDepositorInsertCodon optimized VPS13D (VPS13D Human)
UseTagsnoneExpressionMammalianMutationPromoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-R60f-BFP
Plasmid#186608PurposeEncodes human Reticulon 2 disease mutant R60f fused to BFPDepositorInsertHuman Reticulon 2 mutant R60f (RTN2 Human)
UseTagsBFPExpressionMammalianMutationc.178insC (R60f)PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_1
Plasmid#190703PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 1
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9.scr_3
Plasmid#190705PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 3
UseCRISPRTagsExpressionInsectMutationPromoterDrosophila U6Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-sgRNA-Target1 (MpRTN1)
Plasmid#186295PurposeBinary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)DepositorInsertsgRNA-Target1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2378-Tier1-PhCMV-KDEL
Plasmid#169479PurposeTier-1 vector encoding PhCMV-driven ER-retention signal KDEL (PhCMV-KDEL-pA).DepositorInsertPCMV-driven ER retention signal
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-TREX1 g3 Cas9-T2A-mCherry
Plasmid#164252PurposeTranscription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cellsDepositorInsertsgTREX1
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBz118_His_CsoS2
Plasmid#140841PurposeHalothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backboneDepositorInsertCsoS2
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz54_polyPro_polar
Plasmid#140847PurposeSame as pLz47 but with 20 polar amino acids instead of N*- peptideDepositorInsertpolyproline helix with polar residues
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz58_polyPro_CsoS2_CTP
Plasmid#140850PurposeSame as pLz47 but with C-terminal peptide (CTP) instead of N*- peptideDepositorInsertpolyproline helix with CsoS2 C-terminal peptide
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz33_CsoS2_NTD_GFP
Plasmid#140861PurposeHis-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFPDepositorInsertCsoS2 NTD fused to GFP
UseTagsHexahistidine tag and Superfolder GFPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz55_polyPro_random_Npeptide
Plasmid#140846PurposeSame as pLz47 but with randomized N*-peptide sequenceDepositorInsertpolyproline helix with randomized CsoS2 N-peptide
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz56_polyPro_CsoS2_MR_r1
Plasmid#140848PurposeSame as pLz47 but with MR repeat 1 instead of N*-peptideDepositorInsertpolyproline helix with CsoS2 MR repeat 1
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz57_polyPro_CsoS2_CTD_r8
Plasmid#140849PurposeSame as pLz47 but with CTD repeat 8 instead of N*-peptideDepositorInsertpolyproline helix with CsoS2 CTD repeat 8
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST
Plasmid#129264PurposeExpress soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cellsDepositorInsertMac_Q139D_D150N_E258V-HaloTag-ST
UseTagsExpressionMammalianMutationQ139D,D150N,E258VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST
Plasmid#129263PurposeExpress soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cellsDepositorInsertQuaAr3_Q95D_H106N_E214V-HaloTag-ST
UseTagsExpressionMammalianMutationQ95D, H106N, E214VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST
Plasmid#129260PurposeExpress soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cellsDepositorInsertAce1_Q89D_D100N_E206V-HaloTag-ST
UseTagsExpressionMammalianMutationQ89D, D100N, E206VPromoterCAGAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB-puro-mU6-RtcAshRNA1
Plasmid#126613PurposeExpresses shRNA against human RtcA under mouse U6 promoterDepositorInsertRtcA shRNA-1
UseRNAiTagsExpressionMutationPromotermouse U6 promoterAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB-puro-mU6-RtcAshRNA2
Plasmid#126614PurposeExpresses shRNA against human RtcA under mouse U6 promoterDepositorInsertRtcA shRNA-2
UseRNAiTagsExpressionMutationPromoterMouse U6 promoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CeNL(Ca2+)_110μ-pcDNA3
Plasmid#111929PurposeCyan color bright luminescent calcium indicator with low affinityDepositorInsertCeNL(Ca2+)_110μ
UseTagsExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Sec31a S527/799/1165A #31#907
Plasmid#42121DepositorInsertSec31a (SEC31A Human)
UseTagsFLAGExpressionMammalianMutationS527A S799A S1165APromoterCMVAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) F234C
Plasmid#170644PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationC277A, F234CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421) V304C
Plasmid#170645PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
UseTagsExpressionBacterialMutationC277A, V304CPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-20nm-EBFP2
Plasmid#231185PurposeExpresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nmDepositorInsert20nm-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-20nmEML-mRFP
Plasmid#231181PurposeExpresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vectorDepositorInsert20nmEML-mRFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-10nm-EBFP2
Plasmid#231184PurposeExpresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nmDepositorInsert10nm-EBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-30nmEML-mRFP
Plasmid#231182PurposeExpresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vectorDepositorInsert30nmEML-mRFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLz37_CB_operon_CsoS2_N1-N4
Plasmid#140856PurposeHalothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.DepositorInsertCbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Lime
Plasmid#191255PurposeBacterial expression plasmids for pH sensitive fluorescent protein LimeDepositorInsertLime
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF6-myc-TBC1D15
Plasmid#79148Purposemammalian expression of TBC1D15DepositorInsertTBC1D15 (Tbc1d15 Mouse)
UseTagsHis, Myc, and V5ExpressionMammalianMutationS616N (please see depositor comment below)PromoterEF-1alphaAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab7Q67L
Plasmid#169038PurposeExpress human constitutively active form of Rab7a(Q67L) in mammalian cellsDepositorInsertRab7a (RAB7A Human)
UseTagsacGFP1ExpressionMammalianMutationQ67LPromoterCMVAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBz15_Strep_Rubisco
Plasmid#140839Purposewild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backboneDepositorInsertForm IA Rubisco; CbbL, CbbS
UseTagsStrep IIExpressionBacterialMutationPromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_membrane_EGFP
Plasmid#139402Purposein vitro transcription for mRNADepositorInsertmembrane-EGFP
UseIn vitro transcriptionTagsEGFPExpressionMutationPromoterT3Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam p53 wt
Plasmid#16434DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only