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AiP12724 - pAAV-ExE0638m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#234624

Purpose

ExE0638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12725 - pAAV-ExE0639m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#234625

Purpose

ExE0639m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12729 - pAAV-ExE0580m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#234626

Purpose

ExE0580m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1605 - pAAV-AiE2272m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230538

Purpose

AiE2272m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1272 - pAAV-AiE2088m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214478

Purpose

AiE2088m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pBW2272_pCAG-PV1-FlpO-N27-L1-FRB-NLS-BGHpA

Plasmid

#108755

Purpose

Expresses N-terminus to aa27 of Flp recombinase fused to FRB CID

Depositor

Wilson Wong

Publication

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa27

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89055

Purpose

PV2 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89053

Purpose

PV1 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA

Plasmid

#87580

Purpose

PV1 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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IFI16-(192-393)-HinA-E222A-E272A-E381A

Plasmid

#35061

Depositor

Cheryl Arrowsmith

Publication

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Tags

His

Mutation

Contains HinA domain: aa 192-393; E222A, E272A, a…

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pLJC2-UMPS-3xFLAG

Plasmid

#87975

Purpose

3rd generation lentiviral vector for Expression of UMPS in mammalian cells

Depositor

David Sabatini

Publication

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

UMPS (UMPS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET23MutS-F39A

Plasmid

#13044

Depositor

Peggy Hsieh

Publication

Malkov et al J Biol Chem. 1997 Sep 19. 272(38):23811-

Insert

MutS

Expression

Bacterial

Mutation

Phe39Ala mutation Full length

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pR-BAM

Plasmid

#22649

Depositor

Lynn Hudson

Publication

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1L (Myt1l Rat)

Use

Cloning and rna synthesis

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pLJM1-Rap2A-3xFLAG

Plasmid

#87973

Purpose

Expression of Rap2A in mammalian cells

Depositor

David Sabatini

Publication

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Cav (1-61)

Plasmid

#22446

Depositor

William Sessa

Publication

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1 (1-61)

Tags

GST

Expression

Bacterial

Available since

Jan. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pJC2729 - pLenti-X1-EF1A-HA-FBXO31(∆F-box, D334N)-PGK-Neo

Plasmid

#250261

Purpose

cDNA expression of affinity-tagged FBXO31 (HA-FBXO31(∆F-box, D334N)).

Depositor

Jacob Corn

Publication

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Insert

FBXO31 (FBXO31 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

∆F-box, D334N

Promoter

EF-1a

Available since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Wing

Plasmid

#124552

Purpose

Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

The 5 residues making up the "wing" in …

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3/S3

Plasmid

#124627

Purpose

Chimeric ETS domain: H3/S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 237 to 241 replaced with corresponding s…

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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