We narrowed to 4,194 results for: 424

1. pX330-MGAT1-KO

   Plasmid

   #80009

   Purpose

   gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   MGAT1 (MGAT1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-AMVpl-δWT-nAchR

   Plasmid

   #97188

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Delta nicotinic acetylcholine receptor (Chrnd Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX Nsp2-SH2

   Plasmid

   #46460

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Nsp2 (BCAR3 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 144-259

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPN066

   Plasmid

   #91601

   Purpose

   Express sgRNA targeting human DPP4

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DPP4 (DPP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pPN067

   Plasmid

   #91602

   Purpose

   Express sgRNA targeting human DPP4

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   DPP4 (DPP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Oct. 12, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. MAC-DPP4

   Plasmid

   #172416

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   Dipeptidyl peptidase 4 (DPP4 Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA5-Flag-EAF2

   Plasmid

   #49251

   Purpose

   Mammalian expression of flag-tagged human EAF2

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   EAF2 (EAF2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

8. TFORF0348

   Plasmid

   #143399

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXB2 (FOXB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAMK2B gRNA (BRDN0001145558)

   Plasmid

   #77743

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CAMK2B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CAMK2B (CAMK2B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAMK2B gRNA (BRDN0001148038)

    Plasmid

    #77744

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CAMK2B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CAMK2B (CAMK2B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAMK2B gRNA (BRDN0001148102)

    Plasmid

    #77742

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CAMK2B

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CAMK2B (CAMK2B Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBabe_puro_DEST_Flag_MDS1

    Plasmid

    #45258

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MDS1 (MECOM, MDS1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pBa-FRB-3myc-KIF1A tail 391-1698

    Plasmid

    #64286

    Purpose

    KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    KIF1A (Kif1a, Kif1a Mouse, Rat)
    

    Use

    Tags

    3myc and FRB

    Expression

    Mammalian

    Mutation

    aa 391-1698, 5'end mostly matches rat, 3…

    Promoter

    chicken Beta Actin

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORANGE GFP-CACNA1E KI

    Plasmid

    #131481

    Purpose

    Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacna1e, Cacna1e Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_DIRC2

    Plasmid

    #131880

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    DIRC2 (DIRC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDONR221-DIRC2_STOP

    Plasmid

    #161046

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    DIRC2 (DIRC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pDONR223-CAMK2B

    Plasmid

    #23820

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CAMK2B (CAMK2B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pWZL Neo Myr Flag CAMK2B

    Plasmid

    #20439

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    CAMK2B (CAMK2B Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

19. Split 'n coexpress CUL3-RBX1

    Plasmid

    #52293

    Purpose

    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Inserts

    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    

    Use

    Tags

    GST, IRES, and TEV

    Expression

    Bacterial

    Mutation

    contains aa 1-309, contains aa 310-768, and conta…

    Promoter

    IRES and tac

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pETDest

    Plasmid

    #124245

    Purpose

    Allows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26

    Depositor

    Attila Fehér

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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