We narrowed to 1,456 results for: U6 promoter

1. pCAT007

   Plasmid

   #171634

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLQ1371-sgGAL4

   Plasmid

   #121514

   Purpose

   sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgGAL4
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJRH056

   Plasmid

   #171637

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-tdTomato
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAT024

   Plasmid

   #171636

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BFP
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPR/eCas9-GPIDAF

   Plasmid

   #213710

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPR/eCas9-GPIBY55

   Plasmid

   #213709

   Purpose

   To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3XFLAG and Twin-strep-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

   Plasmid

   #68356

   Purpose

   -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
   FlpO recombinase
   

   Use

   CRISPR

   Tags

   mTurquoise2 (BFP)

   Expression

   Mammalian

   Mutation

   Promoter

   Synthetic Probasin ARRx2 and U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-SAM-Puro

   Plasmid

   #198713

   Purpose

   For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   EF1a-MPH-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-SAM-Cre

   Plasmid

   #198714

   Purpose

   For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   Pgk-MPH-Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pgk and U6

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. plenti guide Thy1.1

    Plasmid

    #128063

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GG-EBNA-OSK2M2L1-PP

    Plasmid

    #102902

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OSK2M2L1-gRNAs-PGK-puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJRH051

    Plasmid

    #171625

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ1371-sgSTAT3-1

    Plasmid

    #121425

    Purpose

    sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgSTAT3-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid

    #190597

    Purpose

    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    EGFP - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid

    #190598

    Purpose

    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    mCherry - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKSB-AeU6-3gRNA

    Plasmid

    #183914

    Purpose

    gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    gRNA scaffold for protospacer cloning into BbsI sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti U6 promoter (AAEL017763)

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-shCTRL

    Plasmid

    #181875

    Purpose

    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    control shRNA with no known targets in the mouse genome
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge