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Plasmid#236163PurposePlasmid encoding the SCN1A-374 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-374-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW171 CMV-TO-UTRN-43ZF-VP64 (FLP-IN)
Plasmid#236161PurposePlasmid encoding the UTRN-43 zinc finger array attached by a GS linker to the VP64 transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUTRN-43ZF-VP64
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF1 CMV-TO-SCN1A-377-NZF
Plasmid#236152PurposePlasmid encoding the SCN1A-377 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-377-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW418 PGK-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)
Plasmid#236155PurposePlasmid encoding the IKZF1-derived portion of the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoterDepositorInsertIKZF1(2-3)-deimmunLink-NZF
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW410 IKZF1tar x8-H2B-GFP
Plasmid#236156PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with eight IKZF1 binding sit…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW368 CMV-TO-UtroUpZF-deImmunLink-NZF(FLP-IN)
Plasmid#236154PurposePlasmid encoding the UtroUp zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUtroUpZF-deImmunLink-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW394 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8-FKBP (FLP-IN)
Plasmid#236158PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a GS linker to FKBP1A, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF250(1-4)-FKBP
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF6 CMV-TO-SCN1A-114-NZF
Plasmid#236167PurposePlasmid encoding the SCN1A-114 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-114-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF7 CMV-TO-SCN1A-140-NZF
Plasmid#236168PurposePlasmid encoding the SCN1A-140 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-140-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF8 CMV-TO-SCN1A-475-NZF
Plasmid#236169PurposePlasmid encoding the SCN1A-475 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-475-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB
Plasmid#236140PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF250(1-4)-deImmunLink-ZIK1KRAB
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW425 CMV-TO-ZNF35(5-8)-IKZF1(2-3)-realDeImmLink-ZIK1KRAB
Plasmid#236142PurposePlasmid encoding the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-IKZF1(2-3)-deImmunLink-ZIK1KRAB
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PAC_E83_LOV_eGFP
Plasmid#231574PurposeThis plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue lightDepositorInsertPAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET16b-RPA4/His14 (MSW#949)
Plasmid#208075PurposeExpression of human Replication Protein A 4 and His-tagged RPA3 in E.coliDepositorInsertRPA4, RPA14(His-tagged)
UseTags10xHis tag on RPA14ExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p11d-RPA70/14 (MSW#134)
Plasmid#208076PurposeExpression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tagDepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW377 - pTMO5
Plasmid#115988Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpTMO5
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3
Plasmid#107301PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP and TALE
UseCRISPRTagsFKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-Avr-HPRT-RIGHT
Plasmid#107272PurposeExpresses TALEN (right) targeting HPRT exon3DepositorInsertHPRT1_B Avr-TALEN (right)
UseTALENTags3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only