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Plasmid#141029PurposeReporter integrationDepositorInsertmCerulean
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
289aa Met133, 138, 186Ileu ELL2
Plasmid#127272PurposeExpresses 289aa human ELL2 with Met133, 138, 186IleuDepositorInsertELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
UseTagsExpressionMammalianMutationMet133, 138, 186 Ileu, cut with XbaI blunt fillinPromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD05 S. castellii telomerase RNA KBS
Plasmid#61894PurposePlasmid for run-off in vitro transcription of Saccharomyces castellii KBS RNADepositorInsertTelomerase RNA hairpin
UseTagsExpressionMutationPromoterT7Available sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Anti-Pan-Neurofascin (extracellular) [A12/18R]
Plasmid#177438PurposeMammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.DepositorInsertanti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-yellow pre-eGRASP(p30)
Plasmid#111587PurposeAn AAV vector that expresses yellow pre-eGRASP(p30) under the CaMKIIa promoter.DepositorInsertyellow pre-eGRASP(p30)
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TIWB-yellow pre-eGRASP(p30)
Plasmid#111588PurposeAn AAV vector that expresses yellow pre-eGRASP(p30) under the tetO promoter.DepositorInsertyellow pre-eGRASP(p30)
UseAAVTagsExpressionMutationPromotertetOAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CWB-yellow pre-eGRASP(p32)
Plasmid#111580PurposeAn AAV vector that expresses yellow pre-eGRASP(p32) under the CaMKIIa promoter.DepositorInsertyellow pre-eGRASP(p32)
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX408 Francisella tularensis subsp. novicida Cas9
Plasmid#68705PurposeFrancisella tularensis subsp. novicida Cas9DepositorInsertFtCas9
UseCRISPRTagsHA-NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TIWB-yellow pre-eGRASP(p32)
Plasmid#111582PurposeAn AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.DepositorInsertyellow pre-eGRASP(p32)
UseAAVTagsExpressionMutationPromotertetOAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX379 Legionella pneumophila str. Paris Cas9
Plasmid#68316PurposeLegionella pneumophila str. Paris Cas9DepositorInsertLpCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 (Yellowhammer)
Plasmid#212402PurposeEncodes SARS-CoV-2 spike L5F,D614G,Q677H for pseudovirus productionDepositorInsertSARS-CoV-2 Spike L5F,D614G,Q677H (S Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationL5F,D614G,Q677HPromoterCAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-dellinker-siRNAres-V5His6_E
Plasmid#146234PurposeInsect Expression of DmSMG6-dellinker-siRNAresDepositorInsertDmSMG6-dellinker-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA tagged SphI ELL2 M133, 138, 186ILeu
Plasmid#127277PurposeExpresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains HA tag near C-terminusDepositorInsertELL2 with Met 133, 138, 186 to Ileu (ELL2 Human)
UseTagsExpressionMammalianMutationMet 133, 138, 186 to Ileu; HA inserted at SphI af…PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged mid ELL2 M133, 138, 186ILeu
Plasmid#127276PurposeExpresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt functionDepositorInsertELL2 (ELL2 Human)
UseTagsExpressionMammalianMutationM133, 138, 186 to Ileu, HA tag at XbaI after Met …PromoterpEF1aAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)
Plasmid#49069PurposeExpresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC563S,C568S,C577S,C582S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-15b(+) Mortierella verticillate (delta prenylation motif)
Plasmid#170788PurposeExpresses pET-15b(+) Mortierella verticillate (delta prenylation motif)DepositorInsertMortierella verticillate PRL
UseTagsN-terminal His-tagExpressionBacterialMutationDeleted nucleotides coding for 4 C-terminal amino…PromoterAmpR, lacIAvailable sinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPELL-pBabe-Vav2(DPZ) (Component 2-T2AP2A-Component 1)
Plasmid#170488PurposeSPELL Vav2(DH-PH-ZnF). Two components cloned into the same plasmid with T2A/P2A sequence. Component 2-T2A-P2A-Component 1DepositorInsertFRB, Vav2(C-lobe), iFKBP, Vav2(N-lobe), mCherry
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2 Met133, 138, 186 to Ileu
Plasmid#127269PurposeFor in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 IleuDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu HA tag after M186PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2 Met133, 138, 186 to Ileu
Plasmid#127271PurposeFor in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th MetDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu with HA tag after 8th MetPromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)
Plasmid#49080PurposeExpresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC791S, C820S, C1052S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only