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Showing: 341 - 360 of 16022 results

  1. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197065
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    I3-01
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197065-plasmid-map-sequence-id-402601

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  2. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197064
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197064-plasmid-map-sequence-id-402605

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  3. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197062
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197062-plasmid-map-sequence-id-402606

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  4. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197063
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197063-plasmid-map-sequence-id-408409

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  5. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

    Plasmid
    #187327
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187327-plasmid-map-sequence-id-373284

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  8. pFUSEss-CHIg-mM-Asn209Asp_M18

    Plasmid
    #91781
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Asp (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Asp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91781-plasmid-map-sequence-id-181014

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  9. pFUSEss-CHIg-mM-Asn209Ala_M18

    Plasmid
    #91733
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    91733-plasmid-map-sequence-id-180936

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  10. pFUSEss-CHIg-mM-Arg210Ala_M18

    Plasmid
    #91734
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arg210 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91734-plasmid-map-sequence-id-194569

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  11. pFUSEss-CHIg-mM-Asn209Arg_M18

    Plasmid
    #91740
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Arg (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    91740-plasmid-map-sequence-id-191795

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  12. pFUSEss-CHIg-mM-Asn209Ile_M18

    Plasmid
    #91746
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ile (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ile (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91746-plasmid-map-sequence-id-190060

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  13. pFUSEss-CHIg-mM-Asn209Val_M18

    Plasmid
    #91756
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Val (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Val (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    91756-plasmid-map-sequence-id-190059

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  14. pFUSEss-CHIg-mM-Asn209Tyr_M18

    Plasmid
    #91755
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Tyr (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Tyr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91755-plasmid-map-sequence-id-182657

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  15. pFUSEss-CHIg-mM-Asn209Thr_M18

    Plasmid
    #91753
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Thr (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Thr (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91753-plasmid-map-sequence-id-182656

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  16. pFUSEss-CHIg-mM-Lys208Ala_M18

    Plasmid
    #91732
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys208Ala (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lys208 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91732-plasmid-map-sequence-id-182642

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  17. pFUSEss-CHIg-mM-Asn209Trp_M18

    Plasmid
    #91754
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Trp (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Trp (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91754-plasmid-map-sequence-id-181130

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  18. pFUSEss-chimeric-IgM-mouse/human_M18

    Plasmid
    #91738
    Purpose
    Expression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 203-239 (EU numbering) were exchanged to…
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91738-plasmid-map-sequence-id-181015

    Enlarge

  19. pFUSEss-CHIg-mM-Asn209Ser_M18

    Plasmid
    #91752
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Ser (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Ser (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91752-plasmid-map-sequence-id-181013

    Enlarge

  20. pFUSEss-CHIg-mM-Asn209Pro_M18

    Plasmid
    #91751
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Pro (EU numbering).
    Depositor
    Joanna Bereta
    Article
    Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn209 changed to Pro (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    91751-plasmid-map-sequence-id-181012

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Showing: 341 - 360 of 16022 results